Proteintech

RPL11 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

RPL11 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of BxPC-3 cells using 16277-1-AP (RPL11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

RPL11 Antibody in Western Blot (WB) — Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16277-1-AP (RPL11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

16277-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 8 publications 8 publications
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	Assay-dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPL11 Fusion Protein Ag9356 (1-177 aa encoded by BC018970) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.21 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MADQGEKEN PMRELRIRKL CLNICVGESG DRLTRAAKVL EQLTGQTPVF SKARYTVRSF GIRRNEKIAV HCTVRGAKAE EILEKGLKVR EYELRKNNFS DTGNFGFGIQ EHIDLGIKYD PSIGIYGLDF YVVLGRPGFS IADKKRRTGC IGAKHRISKE EAMRWFQQKY DGIILPGK (1-177 aa encoded by BC018970)

Target Information

Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of Proteostasis Is a Pathomechanism in Cockayne Syndrome.

Cell reports

Alupei MC,Maity P,Esser PR,Krikki I,Tuorto F,Parlato R,Penzo M,Schelling A,Laugel V,Montanaro L,Scharffetter-Kochanek K,Iben S

Tue May 08 00:00:00 EDT 2018

Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription.

PloS one

Assfalg R,Alupei MC,Wagner M,Koch S,Gonzalez OG,Schelling A,Scharffetter-Kochanek K,Iben S

Fri Sep 15 00:00:00 EDT 2017

FGFR2 mutations in bent bone dysplasia syndrome activate nucleolar stress and perturb cell fate determination.

Human molecular genetics

Neben CL,Tuzon CT,Mao X,Lay FD,Merrill AE

Fri Sep 01 00:00:00 EDT 2017

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Current biology : CB

Jiang L,Li T,Zhang X,Zhang B,Yu C,Li Y,Fan S,Jiang X,Khan T,Hao Q,Xu P,Nadano D,Huleihel M,Lunenfeld E,Wang PJ,Zhang Y,Shi Q

Mon May 22 00:00:00 EDT 2017

Partial Loss of Rpl11 in Adult Mice Recapitulates Diamond-Blackfan Anemia and Promotes Lymphomagenesis.

Cell reports

Morgado-Palacin L,Varetti G,Llanos S,Gómez-López G,Martinez D,Serrano M

Tue Oct 27 00:00:00 EDT 2015

Non-genotoxic activation of p53 through the RPL11-dependent ribosomal stress pathway.

Carcinogenesis

Morgado-Palacin L,Llanos S,Urbano-Cuadrado M,Blanco-Aparicio C,Megias D,Pastor J,Serrano M

Mon Dec 01 00:00:00 EST 2014

The ribosomal protein S26 regulates p53 activity in response to DNA damage.

Oncogene

Cui D,Li L,Lou H,Sun H,Ngai SM,Shao G,Tang J

Thu Apr 24 00:00:00 EDT 2014

Regulation of Actinomycin D induced upregulation of Mdm2 in H1299 cells.

DNA repair

Li L,Cui D,Zheng SJ,Lou H,Tang J

Wed Feb 01 00:00:00 EST 2012

Defective ribosome assembly in Shwachman-Diamond syndrome.

Blood

Wong CC,Traynor D,Basse N,Kay RR,Warren AJ

Thu Oct 20 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 60S ribosomal protein L11; cell growth-inhibiting protein 34; CLL-associated antigen KW-12; Large ribosomal subunit protein uL5; RP11-223J15.3

Gene Aliases: 2010203J19Rik; DBA7; GIG34; L11; RPL11

UniProt ID: (Human) P62913, (Mouse) Q9CXW4, (Rat) P62914

Entrez Gene ID: (Human) 6135, (Mouse) 67025, (Rat) 362631

Molecular Function: ribosomal protein