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RPL24 Antibody (PA5-89707) in WB
Western blot analysis of RPL24 in extracts of various cell lines. Samples were incubated with RPL24 Polyclonal antibody (Product # PA5-89707) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-89707
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 38-157 of human RPL24
Conjugate
Unconjugated
Form
liquid
Concentration
1.10 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2805738
Product Specific Information
Immunogen sequence: SAFLSKRNPR QINWTVLYRR KHKKGQSEEI QKKRTRRAVK FQRAITGASL ADIMAKRNQK PEVRKAQREQ AIRAAKEAKK AKQASKKTAM AAAKAPTKAA PKQKIVKPVK VSAPRVGGKR; Positive Samples: HT-29, HepG2, HeLa
Target Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L24E family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as ribosomal protein L30 because the encoded protein shares amino acid identity with the L30 ribosomal proteins from S. cerevisiae; however, its official name is ribosomal protein L24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 60S ribosomal protein L24; 60S ribosomal protein L30; belly spot and tail; epididymis secretory protein Li 310; L30; Large ribosomal subunit protein eL24; ribosomal protein L30
Gene Aliases: 0610008L05Rik; Bst; HEL-S-310; L24; RPL24
UniProt ID: (Human) P83731, (Rat) P83732, (Mouse) Q8BP67
Entrez Gene ID: (Human) 6152, (Rat) 64307, (Mouse) 68193
Molecular Function:
ribosomal protein
ribosomal large subunit assembly
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
rRNA processing
translation
translational initiation
SRP-dependent cotranslational protein targeting to membrane
viral transcription
cell-cell adhesion
assembly of large subunit precursor of preribosome
mitotic cell cycle checkpoint
exit from mitosis
optic nerve development
retinal ganglion cell axon guidance
retina development in camera-type eye
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