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Invitrogen
RPL5 Polyclonal Antibody
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FIGURE: 1 / 10
RPL5 Antibody (PA5-27539) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of RPL5 was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RPL5 Polyclonal Antibody (Product # PA5-27539) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-27539
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human RPL5. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain, Rat2.
Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (84%), Pig (97%), Rabbit (98%), Chicken (90%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
RPL5 encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18P family of ribosomal proteins. It is located in the cytoplasm. The protein binds 5S rRNA to form a stable complex called the 5S ribonucleoprotein particle (RNP), which is necessary for the transport of nonribosome-associated cytoplasmic 5S rRNA to the nucleolus for assembly into ribosomes. The protein interacts specifically with the beta subunit of casein kinase II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 60S ribosomal protein L5; Large ribosomal subunit protein uL18; protein phosphatase 1, regulatory subunit 135
Gene Aliases: DBA6; L5; MSTP030; PPP1R135; RPL5; U21RNA
UniProt ID: (Human) P46777, (Rat) P09895, (Mouse) P47962
Entrez Gene ID: (Human) 6125, (Rat) 81763, (Mouse) 100503670
ribosomal large subunit assembly
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
rRNA processing
translation
translational initiation
SRP-dependent cotranslational protein targeting to membrane
viral transcription
ribosomal large subunit biogenesis
positive regulation of phosphatase activity
cellular response to inorganic substance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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