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RPL7 Antibody (A300-740A) in WB
Detection of human and mouse RPL7 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RPL7 antibody BL2443 (Product # A300-740A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate (B). RPL7 was also immunoprecipitated by rabbit anti-RPL7 antibody BL2444 (Product # A300-741A). Detection: Chemiluminescence with exposure times... View More
Product Details
A300-740A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 75 and 125 of Human Ribosomal Protein 7.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat, Chicken, Bovine and Orangutan
Target Information
NIP7 contains 1PUA domain and belongs to the NIP7 family. It may play a role in 60S ribosomal subunit synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 60S ribosomal protein L7; Large ribosomal subunit protein uL30
Gene Aliases: humL7-1; L7; RPL7; Rpl7a; Surf-3
UniProt ID: (Human) P18124, (Mouse) P14148
Entrez Gene ID: (Human) 6129, (Mouse) 19989
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
cytoplasmic translation
rRNA processing
translation
translational initiation
SRP-dependent cotranslational protein targeting to membrane
viral transcription
ribosomal large subunit biogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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