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RPP30 Antibody (PA5-97075) in IHC (P)
Immunohistochemistry analysis of RPP30 in paraffin-embedded human lung cancer. Samples were incubated with RPP30 Polyclonal antibody (Product # PA5-97075) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-97075
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-268 of human RPP30 (NP_0064041)
Conjugate
Form
Liquid
Concentration
0.72 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2808877
Product Specific Information
Immunogen sequence: MAVFADLDLR AGSDLKALRG LVETAAHLGY SVVAINHIVD FKEKKQEIEK PVAVSELFTT LPIVQGKSRP IKILTRLTII VSDPSHCNVL RATSSRARLY DVVAVFPKTE KLFHIACTHL DVDLVCITVT EKLPFYFKRP PINVAIDRGL AFELVYSPAI KDSTMRRYTI SSALNLMQIC KGKNVIISSA AERPLEIRGP YDVANLGLLF GLSESDAKAA VSTNCRAALL HGETRKTAFG IISTVKKPRP SEGDEDCLPA SKKAKCEG; Positive Samples: HeLa, Jurkat, HT-1080, HepG2; Cellular Location: Nucleus, nucleolus
Target Information
RNase P, which consists of a complex of an RNA species (H1 RNA), POP1 (Processing of Precursor 1), POP5 (Processing of Precursor 5), and at least seven Rpps (including Rpp14, Rpp29, Rpp30 and Rpp38), removes the 5' leader sequences from precursor tRNA molecules. The structurally related RNase MRP plays an essential role in the formation of the 5' end of 5.8S rRNA. Both RNase P and RNase MRP are associated with Th/To ribonucleoproteins; Rpp30 and Rpp38 have specifically been implicated as Th autoantigens which contribute to the autoimmune disease systemic sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ribonuclease P protein subunit p30; ribonuclease P/MRP 30kDa subunit; ribonuclease P/MRP subunit; ribonuclease P2; RNase P subunit 2; RNaseP protein p30
Gene Aliases: RNASEP2; RPP30; TSG15
UniProt ID: (Human) P78346, (Mouse) O88796
Entrez Gene ID: (Human) 10556, (Rat) 685332, (Mouse) 54364
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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