Proteintech

RPS12 Polyclonal Antibody

RPS12 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

RPS12 Antibody (16490-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using RPS12 antibody 16490-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

RPS12 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

RPS12 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16490-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RPS12 Fusion Protein Ag9615 (1-132 aa encoded by BC017321)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody specifically recognizes the 15 kDa RPS12 protein.

Immunogen sequence: MAEEGIAAG GVMDVNTALQ EVLKTALIHD GLARGIREAA KALDKRQAHL CVLASNCDEP MYVKLVEALC AEHQINLIKV DDNKKLGEWV GLCKIDREGK PRKVVGCSCV VVKDYGKESQ AKDVIEEYFK CKK (1-132 aa encoded by BC017321)

Target Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S12E family of ribosomal proteins. It is located in the cytoplasm. Increased expression of this gene in colorectal cancers compared to matched normal colonic mucosa has been observed. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Haploinsufficiency of the essential gene Rps12 causes defects in erythropoiesis and hematopoietic stem cell maintenance.

eLife

Folgado-Marco V,Ames K,Chuen J,Gritsman K,Baker NE

Mon Jun 05 00:00:00 UTC 2023

Amelioration of gamma irradiation-induced salivary gland damage in mice using melatonin.

Journal of pineal research

Ren J,Zhu T,Wang Z,Lu L,Fan S

Fri Sep 01 00:00:00 UTC 2023

Identification of ribosomal protein L9 as a novel regulator of proinflammatory damage-associated molecular pattern molecules.

Molecular biology reports

Watanabe M,Toyomura T,Wake H,Nishinaka T,Hatipoglu OF,Takahashi H,Nishibori M,Mori S

Fri Apr 01 00:00:00 UTC 2022

RNA G-quadruplex structures control ribosomal protein production.

Scientific reports

Varshney D,Cuesta SM,Herdy B,Abdullah UB,Tannahill D,Balasubramanian S

Tue Nov 23 00:00:00 UTC 2021

HumanaFly: high-throughput transgenesis and expression of breast cancer transcripts in Drosophila eye discovers the RPS12-Wingless signaling axis.

Scientific reports

Katanaev VL,Kryuchkov M,Averkov V,Savitsky M,Nikolaeva K,Klimova N,Khaustov S,Solis GP

Thu Dec 03 00:00:00 UTC 2020

On-Site Ribosome Remodeling by Locally Synthesized Ribosomal Proteins in Axons.

Cell reports

Shigeoka T,Koppers M,Wong HH,Lin JQ,Cagnetta R,Dwivedy A,de Freitas Nascimento J,van Tartwijk FW,Ströhl F,Cioni JM,Schaeffer J,Carrington M,Kaminski CF,Jung H,Harris WA,Holt CE

Tue Dec 10 00:00:00 UTC 2019

Downregulation of ribosome biogenesis during early forebrain development.

eLife

Chau KF,Shannon ML,Fame RM,Fonseca E,Mullan H,Johnson MB,Sendamarai AK,Springel MW,Laurent B,Lehtinen MK

Thu May 10 00:00:00 UTC 2018

Downregulation of ribosome biogenesis during early forebrain development.

eLife

Chau KF,Shannon ML,Fame RM,Fonseca E,Mullan H,Johnson MB,Sendamarai AK,Springel MW,Laurent B,Lehtinen MK

Thu May 10 00:00:00 UTC 2018

Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure.

Developmental cell

Chau KF,Springel MW,Broadbelt KG,Park HY,Topal S,Lun MP,Mullan H,Maynard T,Steen H,LaMantia AS,Lehtinen MK

Mon Dec 21 00:00:00 UTC 2015

Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure.

Developmental cell

Chau KF,Springel MW,Broadbelt KG,Park HY,Topal S,Lun MP,Mullan H,Maynard T,Steen H,LaMantia AS,Lehtinen MK

Mon Dec 21 00:00:00 UTC 2015

Recombineering-based dissection of flanking and paralogous Hox gene functions in mouse reproductive tracts.

Development (Cambridge, England)

Raines AM,Adam M,Magella B,Meyer SE,Grimes HL,Dey SK,Potter SS

Mon Jul 01 00:00:00 UTC 2013

Recombineering-based dissection of flanking and paralogous Hox gene functions in mouse reproductive tracts.

Development (Cambridge, England)

Raines AM,Adam M,Magella B,Meyer SE,Grimes HL,Dey SK,Potter SS

Mon Jul 01 00:00:00 UTC 2013

RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector.

International journal of oncology

Chen D,Zhang R,Shen W,Fu H,Liu S,Sun K,Sun X

Wed May 01 00:00:00 UTC 2013

RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector.

International journal of oncology

Chen D,Zhang R,Shen W,Fu H,Liu S,Sun K,Sun X

Wed May 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: 40S ribosomal protein S12; Small ribosomal subunit protein eS12

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Gene Aliases: RPS12; S12

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UniProt ID: (Human) P25398, (Mouse) P63323

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Entrez Gene ID: (Human) 6206, (Rat) 65139, (Mouse) 20042

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Function(s)

structural constituent of ribosome poly(A) RNA binding molecular_function ribosomal protein

Process(es)

nuclear-transcribed mRNA catabolic process, nonsense-mediated decay rRNA processing translation translational initiation SRP-dependent cotranslational protein targeting to membrane viral transcription biological_process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RPS12 Polyclonal Antibody

RPS12 Antibody (16490-1-AP) in ICC/IF

Product Details

16490-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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