Invitrogen

RPS28 Polyclonal Antibody

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RPS28 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis of RPS28 in U2OS cells using RPS28 Polyclonal Antibody (Product # PA5-116787) at a dilution of 1:100. Blue: DAPI for nuclear staining.

RPS28 Antibody in Western Blot (WB) — Western blot analysis of RPS28 using RPS28 Polyclonal Antibody (Product # PA5-116787) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

PA5-116787

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein of human RPS28 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	5.51 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Positive Samples: HepG2, Rat liver

Target Information

TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.