Proteintech

RPS4X Polyclonal Antibody

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RPS4X Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells, using RPS4X antibody 14799-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

RPS4X Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer using 14799-1-AP (RPS4X antibody) at dilution of 1:100 (under 40x lens).

RPS4X Antibody in Western Blot (WB) — Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14799-1-AP (RPS4X antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

RPS4X Antibody in Immunoprecipitation (IP) — IP result of anti-RPS4X (IP:14799-1-AP, 3ug; Detection:14799-1-AP 1:500) with HepG2 cells lysate 3200ug.

Product Details

14799-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RPS4X Fusion Protein Ag6513 (1-263 aa encoded by BC000472) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.23 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MARGPKKHL KRVAAPKHWM LDKLTGVFAP RPSTGPHKLR ECLPLIIFLR NRLKYALTGD EVKKICMQRF IKIDGKVRTD ITYPAGFMDV ISIDKTGENF RLIYDTKGRF AVHRITPEEA KYKLCKVRKI FVGTKGIPHL VTHDARTIRY PDPLIKVNDT IQIDLETGKI TDFIKFDTGN LCMVTGGANL GRIGVITNRE RHPGSFDVVH VKDANGNSFA TRLSNIFVIG KGNKPWISLP RGKGIRLTIA EERDKRLAAK QSSG (1-263 aa encoded by BC000472)

Target Information

Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. RPS4Y2 (ribosomal protein S4, Y-linked 2), RPS4Y1 (ribosomal protein S4, Y-linked 1) and RPS4X (ribosomal protein S4, X-linked) are proteins that contain one S4 RNA-binding domain and exist as components of the 40S ribosomal subunit, indicating a possible role in protein synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Low level of the X-linked ribosomal protein S4 in human urothelial carcinomas is associated with a poor prognosis.

Biomarkers in medicine

Paquet ÉR,Hovington H,Brisson H,Lacombe C,Larue H,Têtu B,Lacombe L,Fradet Y,Lebel M

Tue Nov 17 00:00:00 EST 2015

Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms.

Cell

Mino T,Murakawa Y,Fukao A,Vandenbon A,Wessels HH,Ori D,Uehata T,Tartey S,Akira S,Suzuki Y,Vinuesa CG,Ohler U,Standley DM,Landthaler M,Fujiwara T,Takeuchi O

Thu May 21 00:00:00 EDT 2015

Low expression of the X-linked ribosomal protein S4 in human serous epithelial ovarian cancer is associated with a poor prognosis.

BMC cancer

Tsofack SP,Meunier L,Sanchez L,Madore J,Provencher D,Mes-Masson AM,Lebel M

Sat Jun 22 00:00:00 EDT 2013

Low expression of the X-linked ribosomal protein S4 in human serous epithelial ovarian cancer is associated with a poor prognosis.

BMC cancer

Tsofack SP,Meunier L,Sanchez L,Madore J,Provencher D,Mes-Masson AM,Lebel M

Sat Jun 22 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 40S ribosomal protein S4, X isoform; cell cycle gene 2; ribosomal protein S4, X-linked X-like; ribosomal protein S4X isoform; SCR10; Single copy abundant mRNA protein; single-copy abundant mRNA; Small ribosomal subunit protein eS4

Gene Aliases: CCG2; DXS306; RPS4; Rps4-1; RPS4X; S4; SCAR; SCR10

UniProt ID: (Rat) P62703, (Human) P62701, (Mouse) P62702

Entrez Gene ID: (Rat) 100362640, (Human) 6191, (Mouse) 20102

Molecular Function: ribosomal protein

Function(s)

translation multicellular organism development positive regulation of cell proliferation positive regulation of translation nuclear-transcribed mRNA catabolic process, nonsense-mediated decay rRNA processing translational initiation SRP-dependent cotranslational protein targeting to membrane viral transcription

Process(es)

structural constituent of ribosome poly(A) RNA binding protein binding rRNA binding RNA binding

RPS4X Polyclonal Antibody

Product Details

14799-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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