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RRAS Antibody (PA5-141028) in IHC (P)
Immunohistochemistry analysis of RRAS in paraffin-embedded mouse small intestine tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with RRAS Polyclonal antibody (Product # PA5-141028) using a dilution of 1:800 for 1 hour at r... View More
Product Details
PA5-141028
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human RRAS aa 1-218.
Conjugate
Form
Liquid
Concentration
1.98 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.1% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932480
Target Information
The Ras superfamily of small GTPases includes the Ras, Rho, Arf, Rab, and Ran families. Ras-regulated signal pathways control such as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. These GTPases function as molecular switches that control eukaryotic cell function by cycling between two interconvertible forms, a GDP-bound inactive form and a GTP-bound active form. The binding of GTP leads to a conformational change in the downstream effector-binding domain of the G-protein. The small GTPases are monomeric G-proteins with molecular masses over the range 20-30 kDa. Ras is attached to the cell membrane by prenylation. Mutations in the Ras family of proto-oncogenes (comprising H-Ras, N-Ras and K-Ras) are very common, being found in 20% to 30% of all human tumours. The frequency of RAS mutations is among the highest for any gene in human cancer. K-ras mutations occur frequently in non-small-cell lung, colorectal, and pancreatic carcinomas; H-ras mutations are common in bladder, kidney, and thyroid carcinomas; N-ras mutations are found in melanoma, hepatocellular carcinoma, and hematologic malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: oncogene RRAS; p23; ras family small GTP binding protein R-Ras; Ras-related protein R-Ras
Gene Aliases: AI573426; RRAS
UniProt ID: (Human) P10301, (Mouse) P10833
Entrez Gene ID: (Human) 6237, (Mouse) 20130
leukocyte differentiation
Ras protein signal transduction
negative regulation of cell migration
positive regulation of angiogenesis
regulation of protein kinase B signaling
face morphogenesis
regulation of ERK1 and ERK2 cascade
signal transduction
small GTPase mediated signal transduction
metabolic process
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