Proteintech

RRM1 Polyclonal Antibody

Product Image Gallery

RRM1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using RRM1 antibody 10526-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

RRM1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10526-1-AP (RRM1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

RRM1 Antibody in Western Blot (WB) — Rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10526-1-AP (RRM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RRM1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with .2ug RRM1 antibody (10526-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

RRM1 Antibody in Immunoprecipitation (IP) — IP result of anti-RRM1 (IP:10526-1-AP, 5ug; Detection:10526-1-AP 1:800) with K-562 cells lysate 8000ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 27 publications 27 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:20-1:200

View 2 publications 2 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Xenopus

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RRM1 Fusion Protein Ag0789

Conjugate

Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

RRM1 (Ribonucleotide reductase M1 polypeptide) is one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase, an enzyme which catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. It provides the precursors necessary for DNA synthesis. RRM1 is involved in carcinogenesis, tumor progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: R RRM 1; RI RI RR 1; Ribonucleoside-diphosphate reductase large subunit; Ribonucleoside-diphosphate reductase subunit M1; Ribonucleotide reductase large subunit; ribonucleotide reductase M1 polypeptide; ribonucleotide reductase, R1 subunit; RIR 1

Gene Aliases: R1; RIR1; RnrM1; RR1; RRM1

UniProt ID: (Human) P23921, (Mouse) P07742

Entrez Gene ID: (Human) 6240, (Rat) 685579, (Mouse) 20133

Molecular Function: oxidoreductase reductase metabolite interconversion enzyme