|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 288 of Human RRN3|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-Dependent|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-30872 targets RRN3 in WB and ChIP applications and shows reactivity with Human samples.
The PA5-30872 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 288 of Human RRN3.
Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Curcumin induces oxidation-dependent cell cycle arrest mediated by SIRT7 inhibition of rDNA transcription in human aortic smooth muscle cells.
PA5-30872 was used in immunocytochemistry and western blot to determine if curcumin can diminish/prevent the development of cardiovascular pathologies
|Lewinska A,Wnuk M,Grabowska W,Zabek T,Semik E,Sikora E,Bielak-Zmijewska A||Toxicology letters (233:227)||2015|