Proteintech

RXRA Polyclonal Antibody

View all (17) RXRA antibodies

Questions & Answers

Questions & Answers

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RXRA Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using RXRA antibody 21218-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

RXRA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 40x lens).

RXRA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human kidney using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 10x lens).

RXRA Antibody in Western Blot (WB) — Rat liver tissue were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RXRA Antibody in Western Blot (WB) — MCF7 cells were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

RXRA Antibody in Western Blot (WB) — MCF7 cells were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RXRA Antibody in Immunoprecipitation (IP) — IP result of anti-RXRA (IP:21218-1-AP, 4ug; Detection:21218-1-AP 1:600) with MCF-7 cells lysate 800ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

RXRA Fusion Protein Ag15651

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in liver, also expressed in lung, kidney and heart. It specifically recognizes the 51 kDa human RXRA protein.

Target Information

Retinoic Acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells. The Retinoid X Receptors (RXRs) family (RXR alpha, beta, and gamma), preferentially bind 9-cis -RA and regulate gene transcription by forming heterodimers with a second family of RA receptors. RAs have been suggested to potentially play a therapeutic role in cervical cancer. RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Nuclear receptor subfamily 2 group B member 1; Retinoic acid receptor RXR-alpha; retinoid X nuclear receptor alpha; retinoid X receptor; Retinoid X receptor alpha; retinoid X receptor, alpha; rxr alpha; RXR alpha 1

Gene Aliases: 9530071D11Rik; NR2B1; RXRA; RXRalpha1

UniProt ID: (Human) P19793, (Mouse) P28700

Entrez Gene ID: (Human) 6256, (Rat) 25271, (Mouse) 20181

Molecular Function: C4 zinc finger nuclear receptor

negative regulation of transcription from RNA polymerase II promoter in utero embryonic development liver development maternal placenta development transcription initiation from RNA polymerase II promoter vitamin metabolic process inflammatory response midgut development embryo implantation aging cholesterol metabolic process negative regulation of cell proliferation response to selenium ion bile acid and bile salt transport modulation by virus of host morphology or physiology axon regeneration regulation of myelination response to retinoic acid cellular response to insulin stimulus response to vitamin A response to vitamin D peroxisome proliferator activated receptor signaling pathway camera-type eye development positive regulation of apoptotic process steroid hormone mediated signaling pathway cellular lipid metabolic process response to ethanol positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation by iron retinoic acid receptor signaling pathway protein homotetramerization response to glucocorticoid ventricular cardiac muscle tissue morphogenesis ventricular cardiac muscle cell differentiation cardiac muscle cell proliferation secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development regulation of branching involved in prostate gland morphogenesis positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus placenta development transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter heart development female pregnancy response to nutrient response to organic cyclic compound positive regulation of transcription, DNA-templated heart morphogenesis negative regulation of gene expression cardiac muscle cell differentiation mesenchyme development angiogenesis involved in coronary vascular morphogenesis visceral serous pericardium development

Process(es)

RNA polymerase II regulatory region sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity retinoic acid receptor activity transcription coactivator activity RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding 9-cis retinoic acid receptor activity protein binding zinc ion binding enzyme binding protein domain specific binding chromatin DNA binding protein complex binding vitamin D receptor binding retinoic acid receptor binding sequence-specific DNA binding transcription regulatory region DNA binding retinoic acid-responsive element binding protein heterodimerization activity vitamin D response element binding double-stranded DNA binding transcription factor binding metal ion binding