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Invitrogen
RYBP Polyclonal Antibody
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FIGURE: 1 / 1
RYBP Antibody (PA5-144636) in WB
Western blot analysis of RYBP in different various lysates. Lane 1: SH-SY5Y cell lysate, 10 µg/Lane; Lane 2: Mouse brain tissue lysate, 20 µg/Lane; Lane 3: Rat kidney tissue lysate, 20 µg/Lane; Lane 4: Rat brain tissue lysate, 20 µg/Lane. Predicted band size: 25 kDa. Observed band size: 25 kDa. Exposure time: 30 seconds; 12% SDS-PAGE gel. Primary antibody RYBP polyclonal antibody (Product # PA5-144636) with a dilution of 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilut... View More
Product Details
PA5-144636
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within Human RYBP aa 1-200/228.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: SH-SY5Y cell lysate, mouse brain tissue lysate, rat kidney tissue lysate, rat brain tissue lysate.
Predicted band size: 25 kDa
Subcellular Location: Cytoplasm, nucleus, nucleoplasm.
Target Information
RYBP involves component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites. Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes. May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis. May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1. May bind to DNA. May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APAP-1; Apoptin-associating protein 1; Death effector domain-associated factor; DED-associated factor; RING1 and YY1-binding protein; Ring1 interactor Rybp; YY1 and E4TF1 associated factor 1; YY1 and E4TF1-associated factor 1
Gene Aliases: 2410018J24Rik; AAP1; APAP-1; DEDAF; RYBP; YEAF1
UniProt ID: (Human) Q8N488, (Mouse) Q8CCI5
Entrez Gene ID: (Human) 23429, (Rat) 312603, (Mouse) 56353
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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