Proteintech

Rab18 Polyclonal Antibody

View all (14) RAB18 antibodies

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Questions & Answers

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Rab18 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer using 11304-1-AP (RAB18 antibody) at dilution of 1:100 (under 40x lens).

Rab18 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer using 11304-1-AP (RAB18 antibody) at dilution of 1:100 (under 10x lens).

Rab18 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 11304-1-AP (Rab18 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Rab18 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 11304-1-AP (Rab18 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Rab18 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Rab18 Antibody in Western Blot (WB) — Human testis tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Rab18 Antibody in Western Blot (WB) — Human heart tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Rab18 Antibody in Western Blot (WB) — Human brain tissue were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Rab18 Antibody in Western Blot (WB) — PC-3 cells were subjected to SDS PAGE followed by western blot with 11304-1-AP (RAB18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Rab18 Antibody in Immunoprecipitation (IP) — IP result of anti-RAB18 (IP:11304-1-AP, 3ug; Detection:11304-1-AP 1:300) with mouse testis tissue lysate 4000ug.

Product Details

11304-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 8 publications 8 publications
Immunohistochemistry (IHC)	-	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Artificial Control, Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Rab18 Fusion Protein Ag1841 (1-206 aa encoded by BC015014) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.1% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MDEDVLTTL KILIIGESGV GKSSLLLRFT DDTFDPELAA TIGVDFKVKT ISVDGNKAKL AIWDTAGQER FRTLTPSYYR GAQGVILVYD VTRRDTFVKL DNWLNELETY CTRNDIVNML VGNKIDKENR EVDRNEGLKF ARKHSMLFIE ASAKTCDGVQ CAFEELVEKI IQTPGLWESE NQNKGVKLSH REEGQGGGAC GGYCSVL (1-206 aa encoded by BC015014)

Target Information

The protein encoded by this gene is a member of a family of Ras-related small GTPases that regulate membrane trafficking in organelles and transport vesicles. Knockdown studies is zebrafish suggest that this protein may have a role in eye and brain development. Mutations in this gene are associated with Warburg micro syndrome type 3. Alternatively spliced transcript variants have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Rab18 overexpression promotes proliferation and chemoresistance through regulation of mitochondrial function in human gastric cancer.

OncoTargets and therapy

Wu B,Qi R,Liu X,Qian L,Wu Z

Wed Mar 30 00:00:00 EDT 2022

Rab18 overexpression promotes proliferation and chemoresistance through regulation of mitochondrial function in human gastric cancer.

OncoTargets and therapy

Wu B,Qi R,Liu X,Qian L,Wu Z

Wed Mar 30 00:00:00 EDT 2022

Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells.

Molecular biology of the cell

Jayson CBK,Arlt H,Fischer AW,Lai ZW,Farese RV,Walther TC

Wed Aug 15 00:00:00 EDT 2018

Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells.

Molecular biology of the cell

Jayson CBK,Arlt H,Fischer AW,Lai ZW,Farese RV,Walther TC

Wed Aug 15 00:00:00 EDT 2018

The RAB GTPase RAB18 modulates macroautophagy and proteostasis.

Biochemical and biophysical research communications

Feldmann A,Bekbulat F,Huesmann H,Ulbrich S,Tatzelt J,Behl C,Kern A

Sat May 06 00:00:00 EDT 2017

ENU mutagenesis identifies mice modeling Warburg Micro Syndrome with sensory axon degeneration caused by a deletion in Rab18.

Experimental neurology

Cheng CY,Wu JC,Tsai JW,Nian FS,Wu PC,Kao LS,Fann MJ,Tsai SJ,Liou YJ,Tai CY,Hong CJ

Fri May 01 00:00:00 EDT 2015

MicroRNA-30b/c inhibits non-small cell lung cancer cell proliferation by targeting Rab18.

BMC cancer

Zhong K,Chen K,Han L,Li B

Wed Sep 24 00:00:00 EDT 2014

MicroRNA-30b/c inhibits non-small cell lung cancer cell proliferation by targeting Rab18.

BMC cancer

Zhong K,Chen K,Han L,Li B

Wed Sep 24 00:00:00 EDT 2014

Rab18 and a Rab18 GEF complex are required for normal ER structure.

The Journal of cell biology

Gerondopoulos A,Bastos RN,Yoshimura S,Anderson R,Carpanini S,Aligianis I,Handley MT,Barr FA

Mon Jun 09 00:00:00 EDT 2014

Rab18 binds to hepatitis C virus NS5A and promotes interaction between sites of viral replication and lipid droplets.

PLoS pathogens

Salloum S,Wang H,Ferguson C,Parton RG,Tai AW

Mon Mar 17 00:00:00 EDT 2014

Hepatitis B virus X protein upregulates oncogene Rab18 to result in the dysregulation of lipogenesis and proliferation of hepatoma cells.

Carcinogenesis

You X,Liu F,Zhang T,Li Y,Ye L,Zhang X

Mon Jul 01 00:00:00 EDT 2013

Hepatitis B virus X protein upregulates oncogene Rab18 to result in the dysregulation of lipogenesis and proliferation of hepatoma cells.

Carcinogenesis

You X,Liu F,Zhang T,Li Y,Ye L,Zhang X

Mon Jul 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: GTP-binding protein Rab18; RAB18 small GTPase; Ras-related protein Rab-18

Gene Aliases: AA959686; RAB18; RAB18LI1; WARBM3

UniProt ID: (Human) Q9NP72, (Mouse) P35293, (Rat) Q5EB77

Entrez Gene ID: (Human) 22931, (Mouse) 19330, (Rat) 307039

transport intracellular protein transport small GTPase mediated signal transduction multicellular organism development protein transport Rab protein signal transduction lipid particle organization endoplasmic reticulum tubular network organization eye development brain development metabolic process

Process(es)

GTPase activity protein binding GTP binding GDP binding nucleotide binding