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Invitrogen
Rab4 Polyclonal Antibody
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FIGURE: 1 / 17
Rab4 Antibody (PA3-912) in ICC/IF
Immunofluorescence analysis of RAB4A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with RAB4A Rabbit polyclonal Antibody (Product # PA3-912) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More
Product Details
PA3-912
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Non-human primate,
Rat
Published species
Bovine,
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified, recombinant human Rab4 protein.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-912 detects Rab4 from human and mouse samples. This antibody does not detect other Rab isoforms (Rab1, Rab3, Rab5, or Rab6).
PA3-912 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~24 kDa band representing Rab4 from transfected HeLa cells.
The PA3-912 immunogen is purified recombinant human Rab4 protein.
Target Information
Protein transport. Probably involved in vesicular traffic.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HTLV-1 related endogenous sequence; Ras-related protein Rab-4A; Ras-related small GTP binding protein 4
Gene Aliases: AI848268; HRES-1; HRES-1/RAB4; HRES1; RAB4; RAB4A
UniProt ID: (Human) P20338, (Rat) P05714, (Mouse) P56371
Entrez Gene ID: (Human) 5867, (Dog) 479210, (Rat) 25532, (Mouse) 19341
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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