Invitrogen

Rabex-5 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

1 Reference

View all (7) Rabex-5 antibodies

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Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

Rabex-5 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 6

Rabex-5 Antibody (PA5-21117) in IHC

Immunohistochemistry of RABEX5 in mouse brain tissue with Rabex-5 Polyclonal Antibody (Product # PA5-21117) at 5 µg/mL.

Product Image Gallery

Rabex-5 Antibody in Western Blot (WB) — Western Blot analysis of RABEX5 in human liver tissue lysate with Rabex-5 Polyclonal Antibody (Product # PA5-21117) at (A) 1 and (B) 2 µg/mL.

Rabex-5 Antibody in Immunocytochemistry (ICC/IF) — Figure 8--figure supplement 1. Silencing synaptic activity affects synaptic levels of endosome proteins in Pclo wt/wt and Pclo gt/gt neurons. ( A and B ) Images of Pclo wt/wt ( A ) and Pclo gt/gt ( B ) neurons stained for Synaptophysin and Rab5 with and without TTX treatment. ( C ) Quantification of ( A and B ). Synaptic Rab5 levels do not significantly alter in Pclo wt/wt and Pclo gt/gt synapses upon TTX treatment ( Pclo wt/wt = 1 +- 0.05, n = 391 synapses; Pclo wt/wt (TTX) = 0.73 +- 0.05, n = 482 synapses; Pclo gt/gt = 1.14 +- 0.09, n = 400 synapses; Pclo gt/gt (TTX) = 0.90 +- 0.04, n = 372 synapses; two independent experiments). ( D and E ) Images of Pclo wt/wt ( D ) and Pclo gt/gt ( E ) neurons stained for Synaptophysin and EEA1 with and without TTX treatment. ( F ) Quantification of ( D and E ). In Pclo wt/wt synapses EEA1 levels drop upon TTX treatment ( Pclo wt/wt = 1 +- 0.03, n = 1653 synapse; Pclo wt/wt (TTX) = 0.72 +- 0.03, n = 1887 synapses; four independent experiments). In contrast, EEA1 levels slightly increase in Pclo gt/gt synapses due to TTX treatment ( Pclo gt/gt = 0.68 +- 0.04, n = 918; Pclo gt/gt (TTX) = 0.78 +- 0.03, n = 1352; four independent experiments). ( G and H ) Pclo wt/wt ( G ) and Pclo gt/gt ( H ) neurons stained for Synaptophysin and Rabex5 with and without TTX treatment. ( I ) Quantification of ( G and H ). Rabex5 level at synapses slightly decrease in Pclo wt/wt and Pclo gt/gt neurons upon TTX trea

Rabex-5 Antibody — Figure 8--figure supplement 1. Silencing synaptic activity affects synaptic levels of endosome proteins in Pclo wt/wt and Pclo gt/gt neurons. ( A and B ) Images of Pclo wt/wt ( A ) and Pclo gt/gt ( B ) neurons stained for Synaptophysin and Rab5 with and without TTX treatment. ( C ) Quantification of ( A and B ). Synaptic Rab5 levels do not significantly alter in Pclo wt/wt and Pclo gt/gt synapses upon TTX treatment ( Pclo wt/wt = 1 +- 0.05, n = 391 synapses; Pclo wt/wt (TTX) = 0.73 +- 0.05, n = 482 synapses; Pclo gt/gt = 1.14 +- 0.09, n = 400 synapses; Pclo gt/gt (TTX) = 0.90 +- 0.04, n = 372 synapses; two independent experiments). ( D and E ) Images of Pclo wt/wt ( D ) and Pclo gt/gt ( E ) neurons stained for Synaptophysin and EEA1 with and without TTX treatment. ( F ) Quantification of ( D and E ). In Pclo wt/wt synapses EEA1 levels drop upon TTX treatment ( Pclo wt/wt = 1 +- 0.03, n = 1653 synapse; Pclo wt/wt (TTX) = 0.72 +- 0.03, n = 1887 synapses; four independent experiments). In contrast, EEA1 levels slightly increase in Pclo gt/gt synapses due to TTX treatment ( Pclo gt/gt = 0.68 +- 0.04, n = 918; Pclo gt/gt (TTX) = 0.78 +- 0.03, n = 1352; four independent experiments). ( G and H ) Pclo wt/wt ( G ) and Pclo gt/gt ( H ) neurons stained for Synaptophysin and Rabex5 with and without TTX treatment. ( I ) Quantification of ( G and H ). Rabex5 level at synapses slightly decrease in Pclo wt/wt and Pclo gt/gt neurons upon TTX treatment ( Pclo wt/wt = 1 +- 0.02, n = 2124 punct {TM}

Product Details

PA5-21117

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

Immunohistochemistry (IHC)

5 µg/mL

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 19 amino acid peptide near the center terminus of human RABEX5.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

RRID

AB_11157010

Product Specific Information

A suggested positive control is rat brain tissue lysate.

PA5-21117 can be used with blocking peptide PEP-1231.

Target Information

RABGEF1 forms a complex with rabaptin-5 (RABPT5) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor (GEF) for RAB5.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Critical role for Piccolo in synaptic vesicle retrieval.

eLife

Ackermann F,Schink KO,Bruns C,Izsvák Z,Hamra FK,Rosenmund C,Garner CC

PA5-21117 was used in Immunocytochemistry to study the synaptic function of Piccolo in rats.

Fri May 10 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: RAB guanine nucleotide exchange factor (GEF) 1; Rab5 exchange factor; Rab5 GDP/GTP exchange factor; Rabaptin-5-associated exchange factor for Rab5; Rabex-5; RAP1; Ras negative regulator Rabex-5; Ras negative regulator Rabex-5/Rin2

Gene Aliases: Rab5ef; rabex-5; RABEX5; RABGEF1; RAP1; Rin2

UniProt ID: (Human) Q9UJ41, (Mouse) Q9JM13

Entrez Gene ID: (Human) 27342, (Mouse) 56715, (Rat) 360797

Molecular Function: guanyl-nucleotide exchange factor