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FIGURE: 1 / 1
Radixin Antibody (PA5-90036) in WB
Western blot analysis of Radixin in extracts of various cell lines. Samples were incubated with Radixin Polyclonal antibody (Product # PA5-90036) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-90036
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RDX
Conjugate
Unconjugated
Form
liquid
Concentration
1.48 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2805901
Product Specific Information
Immunogen sequence: MPKPINVRVT TMDAELEFAI QPNTTGKQLF DQVVKTVGLR EVWFFGLQYV DSKGYSTWLK LNKKVTQQDV KKENPLQFKF RAKFFPEDVS EELIQEITQR LFFLQVKEAI LNDEIYCPPE TAVLLASYAV QAKYGDYNKE IHKPGYLAND RLLPQRVLEQ HKLTKEQWEE RIQNWHEEHR GMLREDSMME YLKIAQDLEM; Positive Samples: LO2, HeLa, Jurkat; Cellular Location: Cell membrane, Cleavage furrow, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton
Target Information
Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ESP10; Radixin; radixin isoform
Gene Aliases: AA516625; DFNB24; RDX
UniProt ID: (Human) F5H1A7, (Mouse) P26043
Entrez Gene ID: (Human) 5962, (Rat) 315655, (Mouse) 19684
Molecular Function:
actin or actin-binding cytoskeletal protein
regulation of cell shape
regulation of cell size
positive regulation of gene expression
protein kinase A signaling
microvillus assembly
positive regulation of cell migration
regulation of actin filament bundle assembly
regulation of Rap protein signal transduction
negative regulation of homotypic cell-cell adhesion
negative regulation of GTPase activity
regulation of GTPase activity
apical protein localization
establishment of protein localization
negative regulation of cell size
actin filament capping
establishment of endothelial barrier
establishment of protein localization to plasma membrane
cellular response to thyroid hormone stimulus
cell-cell adhesion
regulation of ruffle assembly
positive regulation of G1/S transition of mitotic cell cycle
regulation of organelle assembly
positive regulation of protein localization to early endosome
positive regulation of cellular protein catabolic process
negative regulation of adherens junction organization
positive regulation of early endosome to late endosome transport
cellular response to platelet-derived growth factor stimulus
barbed-end actin filament capping
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