Invitrogen

Radixin Polyclonal Antibody

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Radixin Antibody in Western Blot (WB) — Western blot analysis of extracts of various cell lines, using RDX Polyclonal antibody (Product # PA5-90036) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 1s.

Product Details

Tested Applications

Dilution

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human RDX

Conjugate

Unconjugated

Form

liquid

Concentration

2.9 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen sequence: MPKPINVRVT TMDAELEFAI QPNTTGKQLF DQVVKTVGLR EVWFFGLQYV DSKGYSTWLK LNKKVTQQDV KKENPLQFKF RAKFFPEDVS EELIQEITQR LFFLQVKEAI LNDEIYCPPE TAVLLASYAV QAKYGDYNKE IHKPGYLAND RLLPQRVLEQ HKLTKEQWEE RIQNWHEEHR GMLREDSMME YLKIAQDLEM; Positive Samples: LO2, HeLa, Jurkat; Cellular Location: Cell membrane, Cleavage furrow, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton

Target Information

Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene was assigned to Xp21.3. Another pseudogene that seemed to lack introns was mapped to 11p by Southern and PCR analyses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ESP10; Radixin; radixin isoform

Gene Aliases: AA516625; DFNB24; RDX

UniProt ID: (Human) P35241, (Mouse) P26043

Entrez Gene ID: (Human) 5962, (Rat) 315655, (Mouse) 19684

Molecular Function: actin family cytoskeletal protein cytoskeletal protein

regulation of cell shape regulation of cell size positive regulation of gene expression protein kinase A signaling microvillus assembly positive regulation of cell migration regulation of actin filament bundle assembly regulation of Rap protein signal transduction negative regulation of homotypic cell-cell adhesion negative regulation of GTPase activity regulation of GTPase activity apical protein localization establishment of protein localization negative regulation of cell size actin filament capping establishment of endothelial barrier establishment of protein localization to plasma membrane cellular response to thyroid hormone stimulus cell-cell adhesion regulation of ruffle assembly positive regulation of G1/S transition of mitotic cell cycle regulation of organelle assembly positive regulation of protein localization to early endosome positive regulation of cellular protein catabolic process negative regulation of adherens junction organization positive regulation of early endosome to late endosome transport cellular response to platelet-derived growth factor stimulus barbed-end actin filament capping

Process(es)

actin binding protein binding protein domain specific binding protein homodimerization activity poly(A) RNA binding protein kinase A binding ATPase binding cadherin binding involved in cell-cell adhesion cytoskeletal protein binding