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RanBP2 Antibody (A301-796A) in WB
Detection of human and mouse RanBP2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP2 antibody (Product # A301-796A; lot A301-796A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A301-796A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1050 and 1100 of Human RAN binding protein 2.
Conjugate
Unconjugated
Form
liquid
Concentration
0.20 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee
Target Information
RanBP2 interacts with the small GTP-binding protein Ran and forms a large multiprotein complex involved in many biological processes such as mRNA processing, nuclear transport, cell cycle control and postmitotic nuclear reassembly. The protein is identified by western blotting as an apparent molecular mass of 360 kD. It is involved in nucleation of microtubule and control of cell growth by interacting with many protein factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 358 kDa nucleoporin; acute necrotizing encephalopathy 1 (autosomal dominant); E3 SUMO-protein ligase RanBP2; Nuclear pore complex protein Nup358; nucleoporin 358; Nucleoporin Nup358; P270; Ran-binding protein 2; RanBP2; RBP2; transformation-related protein 2
Gene Aliases: A430087B05Rik; ADANE; AI256741; ANE1; IIAE3; NUP358; RANBP2; TRP1; TRP2
UniProt ID: (Human) P49792, (Mouse) Q9ERU9
Entrez Gene ID: (Human) 5903, (Mouse) 19386
RNA binding
peptidyl-prolyl cis-trans isomerase activity
protein binding
zinc ion binding
Ran GTPase binding
ligase activity
SUMO transferase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
chaperone
scaffold/adaptor protein
transporter
protein peptidyl-prolyl isomerization
mRNA export from nucleus
tRNA export from nucleus
protein folding
NLS-bearing protein import into nucleus
sister chromatid cohesion
mitotic nuclear envelope disassembly
regulation of glucose transport
viral process
protein sumoylation
viral transcription
gene silencing by RNA
negative regulation of glucokinase activity
intracellular transport of virus
regulation of gluconeogenesis involved in cellular glucose homeostasis
regulation of cellular response to heat
transport
protein transport
intracellular transport
mRNA transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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