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Product Details
PRC0104
| Product Specifications | |
|---|---|
Species |
Rat |
Expression System |
E. coli |
Amino acid sequence |
Rat IL-10 recombinant protein contains 160 amino acids |
Molecular weight |
18.7 kDa |
Class |
Recombinant |
Type |
Protein |
Purity |
>98% by SDS-PAGE and HPLC |
Endotoxin concentration |
<0.1 ng/µg |
Activity |
ED50 ≤ 10 ng/mL; determined by the dose-dependent inhibition of antigen-specific T-cells. |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Purification |
Affinity chromatography |
Storage buffer |
5mM tris, pH 7.2 |
Contains |
no preservative |
Storage conditions |
-20°C |
Product Specific Information
Reconstitution: We recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized rat IL-10 in 5 mM Tris, pH 7.2, to a concentration of 0.1-1.0 mg/mL. Further dilution should be made in medium or buffered solution containing carrier protein, such as PBS with 0.1% BSA.
Storage: This lyophilized preparation is stable at 2°C to 8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2°C to 8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C. Avoid repeated freeze/thaw cycles.
Target Information
Interleukin 10 (IL-10, CSIF) is an anti-inflammatory cytokine mainly produced by macrophages and Th2 cells. IL-10 is an 18.6 kDa protein of 160 amino acid residues that shares over 80% sequence homology with the Epstein-Barr Virus protein (BCRFI). The reported biological activities of IL-10, which maybe interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hyper-sensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including IFN-gamma, TNF-alpha, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells, enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. While human IL-10 can act on murine cells, murine IL-10 cannot act on human cells. Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). IL-10 inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Knockout studies in mice suggest the function of this cytokine as an essential immunoregulator in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1
Gene Aliases: Il-10; Il10; IL10X
UniProt ID: (Rat) P29456
Entrez Gene ID: (Rat) 25325
negative regulation of cytokine production
response to molecule of bacterial origin
negative regulation of cytokine secretion involved in immune response
negative regulation of chronic inflammatory response to antigenic stimulus
positive regulation of B cell apoptotic process
inflammatory response
immune response
aging
response to organic substance
regulation of gene expression
response to activity
response to inactivity
negative regulation of myeloid dendritic cell activation
negative regulation of B cell proliferation
response to lipopolysaccharide
negative regulation of interferon-gamma production
negative regulation of interleukin-12 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
receptor biosynthetic process
response to insulin
response to carbon monoxide
response to drug
negative regulation of tumor necrosis factor biosynthetic process
defense response to bacterium
negative regulation of apoptotic process
negative regulation of growth of symbiont in host
negative regulation of nitric oxide biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JAK-STAT cascade
positive regulation of cytokine secretion
negative regulation of inflammatory response
negative regulation of membrane protein ectodomain proteolysis
positive regulation of sequence-specific DNA binding transcription factor activity
response to glucocorticoid
regulation of sensory perception of pain
branching involved in labyrinthine layer morphogenesis
cellular response to lipopolysaccharide
cellular response to estradiol stimulus
regulation of complement-dependent cytotoxicity
negative regulation of endothelial cell apoptotic process
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