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Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
Assay-dependent | - |
ELISA (ELISA) |
Assay-dependent | - |
Immunoprecipitation (IP) |
Assay-dependent | - |
Control (Ctrl) |
Assay-dependent | - |
Product Specifications | |
---|---|
Species |
Rat |
Expression System |
E. coli |
Molecular weight |
21.7 kDa |
Class |
Recombinant |
Type |
Protein |
Purity |
>98% by SDS-PAGE |
Activity |
ED50 = 0.01 ng/mL; determined by the dose-dependent proliferation of mouse 7TD1 cells. |
Conjugate |
Unconjugated |
Form |
Lyophilized |
Purification |
purified |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
Rat IL-6 exerts activity in the range of 0.01 to 1.0 ng/mL for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water or buffer at pH >7 to a concentration of 100 ng/µL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0°C. Cytokines diluted as indicated above should be divided into aliquots and stored at -70°C. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.
Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: H-IL-6; IL-6; ILN; Interleukin; Interleukin 6 (interferon, beta 2); Interleukin-6; Interleukin6; M-IL-6; R-IL-6
Gene Aliases: Ifnb2; Il-6; Il6; ILg6
UniProt ID: (Rat) P20607
Entrez Gene ID: (Rat) 24498
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