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Invitrogen
Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 615, eBioscience™
Product Details
42-4301-80
| Applications | Tested Dilution |
|---|---|
Immunohistochemistry (Paraffin) (IHC (P)) |
10 µg/mL |
Immunohistochemistry (Frozen) (IHC (F)) |
1-10 µg/mL |
Immunocytochemistry (ICC/IF) |
0.5 µg/mL |
Control (Ctrl) |
Assay-Dependent |
| Product Specifications | |
|---|---|
Host/Isotype |
Rat / IgG1, kappa |
Class |
Control |
Type |
Isotype Control |
Clone |
eBRG1 |
Conjugate |
eFluor™ 615
|
Excitation/Emission Max |
595/614 nm
View spectra
|
Form |
Liquid |
Concentration |
0.2 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2637346 |
Product Specific Information
Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This eBRG1 antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded and frozen tissue sections and immunocytochemistry.
Applications Tested: This eBRG1 antibody has been tested by immunocytochemistry on fixed and permeabilized cells. Isotype control should be used at the same concentration as experimental antibody. This product has not been validated for flow cytometric analysis.
Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.
Excitation: 595 nm; Emission: 615 nm.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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