|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(1) A D K F A A F D D A V E E(14) C of human RbAp48.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA1-869 detects retinoblastoma protein (Rb) associated protein 48 (RbAp48) from human cells.
PA1-869 has been successfully used in IF and Western blot procedures. By Western blot, this antibody detects an ~48 kDa protein representing RbAp48 and a larger ~60 kDa unknown protein from HeLa cell lysate.
The PA1-869 immunogen is a synthetic peptide corresponding to residues M(1) A D K F A A F D D A V E E(14) C of human RbAp48. This sequence is completely conserved between human and mouse RbAp48. This immunizing peptide (Cat. # PEP-084) is available for use in neutralization and control experiments.
Retinoblastoma protein (Rb) associated protein 48 (RbAp48) is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex as are the histone deacetylase proteins (HDACs). RbAp48 shares about 75% sequence identity with the closely related RbAp46, and appears to be functionally related to the yeast MSI1 protein in that overexpression of RbAp48 suppresses heat shock sensitivity in a RAS pathway dependent manner. RbAp48 has also been demonstrated to be up-regulated by the Wilms and quote; tumor suppressor gene product, WT1. Additionally, cells transfected with RbAp48 have shown strong inhibition of growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Atrophin 2 recruits histone deacetylase and is required for the function of multiple signaling centers during mouse embryogenesis.
PA1-869 was used in western blot to investigate the important roles of atrophin proteins during mouse embryogenesis
|Zoltewicz JS,Stewart NJ,Leung R,Peterson AS||Development (Cambridge, England) (131:3)||2004|
Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.
PA1-869 was used in western blot to characterize RbAp46 and study its functional properties
|Qian YW,Lee EY||The Journal of biological chemistry (270:25507)||1995|