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Invitrogen
RbAp48 Polyclonal Antibody
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FIGURE: 1 / 5
RbAp48 Antibody (PA5-30422) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of RbAp48 was performed in A431 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RbAp48 Polyclonal Antibody (Product # PA5-30422) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Product Details
PA5-30422
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human RbAp48. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.28 mg/mL
Amount
28 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, Jurkat, SH-SY5Y, SH-SY5Y nuclear extract.
Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (97%), Xenopus laevis (98%), Chicken (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
RbAp48-11G10 recognizes full length RbAp48, a 48 kDa nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; Chromatin assembly factor 1 subunit C; Chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; Histone-binding protein RBBP4; MSI1 protein homolog; Nucleosome-remodeling factor subunit RBAP48; RBBP-4; Retinoblastoma-binding protein 4; Retinoblastoma-binding protein p48; unnamed protein product; zRBBP4
Gene Aliases: lin-53; NURF55; RBAP48; rbb4; rbb4-2; RBBP4; wu:fb33a09; wu:fb40e10; zgc:55349; zgc:77854
UniProt ID: (Human) Q09028, (Zebrafish) Q6P3H7
Entrez Gene ID: (Human) 5928, (Zebrafish) 321726
DNA replication
chromatin organization
chromatin remodeling
cell cycle
fin regeneration
chromatin assembly
negative regulation of transcription from RNA polymerase II promoter
DNA repair
nucleosome assembly
DNA replication-dependent nucleosome assembly
regulation of transcription, DNA-templated
cellular response to DNA damage stimulus
brain development
negative regulation of cell proliferation
negative regulation of cell migration
negative regulation of transforming growth factor beta receptor signaling pathway
regulation of cell fate specification
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
negative regulation of stem cell population maintenance
positive regulation of stem cell population maintenance
regulation of stem cell differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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