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Recoverin Antibody (PA5-106485) in ICC/IF
Immunofluorescent analysis of Recoverin in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Recoverin polyclonal antibody (Product # PA5-106485) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-106485
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RCVRN(Accession P35243), corresponding to amino acid residues L123-I173.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854154
Product Specific Information
Antibody detects endogenous levels of total Recoverin.
Target Information
Seems to be implicated in the pathway from retinal rod guanylate cyclase to rhodopsin. May be involved in the inhibition of the phosphorylation of rhodopsin in a calcium-dependent manner. The calcium-bound recoverin prolongs the photoresponse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 23 kDa photoreceptor cell-specific protein; cancer associated retinopathy antigen; cancer associated retinopathy protein; Cancer-associated retinopathy protein; guanylate cyclase activator; Protein CAR; Recoverin
Gene Aliases: CAR; RCV1; RCVRN; S-modulin
UniProt ID: (Human) P35243, (Mouse) P34057
Entrez Gene ID: (Human) 5957, (Mouse) 19674, (Rat) 140936
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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