Invitrogen

Reelin Polyclonal Antibody

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Reelin Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of Reelin in human cell line U-2 OS shows localization to focal adhesion sites. Samples were probed using a Reelin Polyclonal Antibody (Product # PA5-65442).

Reelin Antibody in Western Blot (WB) — Western Blot was performed using Anti-Reelin Polyclonal Antibody (Product # PA5-65442) and a 400 kDa band corresponding to Reelin was observed across Hep G2 treated with PTI when compared to HCT 116. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with PTI (1X, for 4 hrs) (Lane 2), HCT 116 (Lane 3), HCT 116 treated with PTI (1X, for 4 hrs) (Lane 4) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Few uncharacterized bands were observed between 50-70 kDa.

Product Details

PA5-65442

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human Reelin View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2663084

Product Specific Information

Immunogen sequence: TFCEPYGPREL ITTGLNTTTA SVLQFSIGSG SCRFSYSDPS IIVLYAKNNS ADWIQLEKIR APSNVSTIIH ILYLPEDAKG ENVQFQWKQE NLRVGEVYE

Highest antigen sequence identity to the following orthologs - mouse 90%, rat 94%.

Target Information

Reelin in an extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Reelin

Gene Aliases: ETL7; LIS2; PRO1598; RELN; RL

UniProt ID: (Human) P78509

Entrez Gene ID: (Human) 5649

Molecular Function: extracellular matrix glycoprotein extracellular matrix protein

cell morphogenesis involved in differentiation neuron migration proteolysis cell adhesion axon guidance central nervous system development brain development long-term memory associative learning glial cell differentiation positive regulation of neuron projection development positive regulation of phosphatidylinositol 3-kinase signaling dendrite development peptidyl-tyrosine phosphorylation spinal cord patterning ventral spinal cord development hippocampus development cerebral cortex tangential migration layer formation in cerebral cortex positive regulation of TOR signaling positive regulation of CREB transcription factor activity reelin-mediated signaling pathway positive regulation of protein kinase activity response to pain positive regulation of peptidyl-tyrosine phosphorylation regulation of behavior modulation of synaptic transmission positive regulation of small GTPase mediated signal transduction positive regulation of synaptic transmission, glutamatergic long-term synaptic potentiation positive regulation of dendritic spine morphogenesis positive regulation of protein tyrosine kinase activity positive regulation of synapse maturation NMDA glutamate receptor clustering postsynaptic density protein 95 clustering receptor localization to synapse lateral motor column neuron migration positive regulation of long-term synaptic potentiation positive regulation of lateral motor column neuron migration regulation of N-methyl-D-aspartate selective glutamate receptor activity positive regulation of excitatory postsynaptic potential positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity

Process(es)

protein serine/threonine/tyrosine kinase activity serine-type peptidase activity metal ion binding lipoprotein particle receptor binding very-low-density lipoprotein particle receptor binding