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|Tested species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues S(21) S M P L C P I D E A I D K K I K Q D C(39) of mouse Resistin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-1050 detects recombinant mouse resistin.
PA1-1050 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~14 kDa protein representing recombinant mouse resistin.
The PA1-1050 immunogen is a synthetic peptide corresponding to residues S(21) S M P L C P I D E A I D K K I K Q D C(39) of mouse Resistin. This peptide (Cat. # PEP-165) is available for use in neutralization and control experiments.
Adipose tissue is an energy reserve in animals and, in nondomestic species, is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin, adipocyte complement related protein of 30 kDa (Acrp30), perilipin, and resistin. Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes. Studies show that in obese, diabetic mice the levels of serum resistin are increased, which has an antagonistic effect on insulin. Additional studies have shown that resistin causes insulin resistance and glucose intolerance in mice.
Resistin gene expression requires C/EBP alpha, which is necessary and sufficient for expression. Studies show that the coactivators CBP and p300, which have been shown to display histone acetyltransferase (HAT) activity, abundantly acetylate the histones at the resistin promoter suggesting their role in activating chromatin for resistin transcription. Numerous PPAR gamma and RXR ligands have been shown to downregulate resistin expression in adipocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
adipose tissue-specific secretory factor; adipose-specific cysteine-rich secreted protein A12-alpha; ADSF; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-; cysteine-rich secreted protein FIZZ3; dominant inhibitory adipocyte-specific secretory factor; found in inflammatory zone 3; resistin; RSN
ADSF; Fizz3; Retn; Rstn; Xcp4