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Rockland
Rheb Polyclonal Antibody
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FIGURE: 1 / 1
Rheb Antibody (600-401-DY7) in WB
Western Blot of Rheb antibody in rat heart cell lysate. Lane 1: Rheb antibody at 2 µg/mL . Lane 2: Rheb antibody at 4 µg/mL . Load: 35 µg per lane. Primary antibody: Rheb antibody at designated concentrations for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 20 kDa, 22 kDa for Rheb . Other band(s): Rheb splice variants and isoforms.
Product Details
600-401-DY7
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Rabbit Rheb polyclonal antibody was prepared from whole rabbit serum produced by repeated immunizations with a 14 amino acid synthetic peptide from the N-terminus of human Rheb.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Rheb encodes a member of the small GTPase superfamily and encodes a lipid-anchored, cell membrane protein with five repeats of the RAS-related GTP-binding region. This protein is vital in regulation of growth and cell cycle progression due to its role in the insulin/TOR/S6K signaling pathway. The protein has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity. Three pseudogenes have been mapped, two on chromosome 10 and one on chromosome 22.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GTP-binding protein Rheb; Ras homolog enriched in brain; Ras homolog enriched in brain 2; RAS-homolog enriched in brain
Gene Aliases: RHEB; Rheb1; RHEB2
UniProt ID: (Human) Q15382, (Mouse) Q921J2, (Rat) Q62639
Entrez Gene ID: (Human) 6009, (Mouse) 19744, (Rat) 26954
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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