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Invitrogen
RhoA Polyclonal Antibody
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FIGURE: 1 / 1
RhoA Antibody (OSR00266W) in ICC/IF
Biomedicines 2022 -
Figure 9 MH regulates endothelial and tumoural cytoskeleton-associated proteins. Confluent monolayers of b.End5 under physiological laminar non-pulsatile shear stress were exposed to 4T1 cells (previously labelled with CellTracker(tm) CMFDA Green Dye) and treated with MH (1 uM), or DMEM (untreated), for 24 h, and the cytoskeleton-associated alterations were evaluated in single and mixed cultures by immunofl... View More
Product Details
OSR00266W
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from aa region 100-150 of human RhoA conjugated to blue carrier protein was used as the antigen.
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Glycerol (1:1) may be added for additional stability.
Reconstitute in 100 µL of sterile water.
The peptide is homologous in many species including rat, mouse, chicken, dog, bovine, frog, zebrafish and orangutan.
Target Information
RhoA regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. It is involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis, and plays an essential role in cleavage furrow formation. RhoA is required for the apical junction formation of keratinocyte cell-cell adhesion, and serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RhoA stimulates PKN2 kinase activity and may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP. It is essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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