|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion full length protein (Human).|
|Storage buffer||0.1M tris glycine, pH 7.4, with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||4 µg|
|Western Blot (WB)||0.5-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28099 detects RhoGDI from human samples. This antibody also cross reacts with D4GDI.
PA1-28099 has been successfully used in immunoprecipitation, and Western blot applications.
The PA1-28099 immunogen is: Fusion full length protein (Human).
GDP - Dissociation inhibitors (GDIs) modulate GTPase activity by binding to small G-proteins and regulating GDP/GTP exchange of inflammatory cell activity, including motility, formation of toxic oxygen metabolies and generation of proinflammatory cytokines. RhoGDI may also be critical for the cellular compartmentalization of GTPases. RhoGDI, which can bind to Rho, Rac, and cdc42, is localized in the cytosol. D4GDI, an abundant hematopoietic cell GDI for the Ras-related Rho family GTPases, is a substrate of the apoptosis protease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.