|Tested species reactivity||Bovine, Sheep|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C S(338) K T E T S Q V A P A(348)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-729 detects rhodopsin from bovine and sheep retina samples. This antibody does not detect rhodopsin from ovine samples
PA1-729 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~36 kDa protein representing rhodopsin from bovine outer rod segment extracts.
The PA1-729 immunizing peptide (Cat. # PEP-174) corresponds to amino acid residues 338-348 from bovine rhodopsin. This peptide is available for use in neutralization and control experiments.
Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein rhodopsin absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble proteins arrestin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Overexpression of rhodopsin alters the structure and photoresponse of rod photoreceptors.
PA1-729 was used in western blot to study how rhodopsin's overexpression affects the structure and photoresponse of rod photoreceptors
|Wen XH,Shen L,Brush RS,Michaud N,Al-Ubaidi MR,Gurevich VV,Hamm HE,Lem J,Dibenedetto E,Anderson RE,Makino CL||Biophysical journal (96:939)||2009|