|Tested species reactivity||Bacteria, Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full-size purified recombinant Rhp51 protein expressed in E. coli.|
|Storage buffer||whole serum|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:2000-1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4968 detects Rhp51 protein in E. coli samples.
PA1-4968 has been successfully used in Western blot, Immunofluorescence, immunocytochemistry and Immunoprecipitation proceedures. It is specific for the ~40 kDa Rhp51 protein. GFP-tagging of Rhp51 protein at either N- or C- terminus inactivates the function of Rhp51, and cannot be used for the study of foci formation.
PA1-4968 immunogen is a highly purified recombinant Rhp51 protein expressed in E. coli.
Rhp51 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of E. coli RecA protein and the Rad51 proteins of eukaryotes. These proteins play a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.