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Agrisera
Rnr1/Ribonucleoside-diphosphate reductase large subunit Polyclonal Antibody
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FIGURE: 1 / 1
Rnr1/Ribonucleoside-diphosphate reductase large subunit Antibody (AS21-4608) in WB
10 µL of total protein from 1 x 10^8 cells of Saccharomyces cerevisiae, extracted with lysis buffer ( 20 µm M Tris, p H 8,50 µm M Ammonium acetate, 2 µm M EDTA, plus protease and phosphatase inhibitor cocktails) containing 10% TCA, and re-suspended and denatured in 1 X LaemmLi buffer for 10 min, were separated on 10% SDS-PAGE and blotted on to nitrocellulose membrane (0.45 µm) for 1.5 h at constant 0.5 Ampe... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
AS21-4608
Product Specifications
Species Reactivity
Yeast
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Two KLH-conjugated synthetic peptides derived from c-terminal of Saccharomyces cerevisiae Rnr1 protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Saccharomyces cerevisiae
Target Information
Rnr1 is a nuclear-encoded, protein-coding gene whose product localizes predominantly to the cytoplasm and functions as the major large (alpha) subunit of the class I ribonucleotide reductase (RNR) complex, with regulated redistribution toward the nucleus under DNA replication stress. Rnr1 is a soluble enzyme subunit that forms an alpha2 catalytic core and associates with the small (beta) subunits to assemble the active alpha2beta2 RNR holoenzyme, in which the small subunit supplies the essential diferric-tyrosyl radical cofactor while Rnr1 provides the catalytic site and allosteric nucleotide-binding sites that control overall activity and substrate specificity. Biologically, Rnr1 catalyzes the rate-limiting reduction of ribonucleoside diphosphates to deoxyribonucleoside diphosphates, thereby sustaining balanced dNTP pools required for nuclear and mitochondrial DNA replication and for DNA repair; consistent with this central role, RNR1 expression is cell-cycle regulated and is also responsive to checkpoint signaling and metabolic cues (including iron availability) that tune dNTP production to replication demand and genotoxic stress.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATRNR1; CLS8; CRINKLY LEAVES 8; defective in pollen organelle DNA degradation 2; DPD2; R1; ribonucleotide reductase 1; RIBONUCLEOTIDE REDUCTASE LARGE SUBUNIT 1
Gene Aliases: CRT7; RIR1; SDS12; YER070W
UniProt ID: (Baker's yeast) P21524
Entrez Gene ID: (Baker's yeast) 856801
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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