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Proteintech
Rubicon Polyclonal Antibody
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Product Details
21444-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Rubicon Fusion Protein Ag13715 (61-290 aa encoded by BC033615)
Conjugate
Form
Liquid
Concentration
0.47 mg/mL
Amount
70.5 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: VAELWLQHS LQYHCLSAQL RPLLGDRQYI RKFYTDAAFL LSDAHVTAML QCLEAVEQNN PRLLAQIDAS MFARKHESPL LVTKSQSLTA LPSSTYTPPN SYAQHSYFGS FSSLHQSVPN NGSERRSTSF PLSGPPRKPQ ESRGHVSPAE DQTIQAPPVS VSALARDSPL TPNEMSSSTL TSPIEASWVS SQNDSPGDAS EGPEYLAIGN LDPRGRTASC QSHSSNAESS S (61-290 aa encoded by BC033615)
Target Information
Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation. Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex. Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Baron; Beclin-1 associated RUN domain containing protein; Rubicon; RUN and cysteine rich domain containing beclin 1 interacting protein; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; run domain Beclin-1 interacting and cystein-rich containing protein; run domain Beclin-1 interacting and cysteine-rich containing protein; Run domain Beclin-1-interacting and cysteine-rich domain-containing protein; rundataxin; similar to mKIAA0226 protein; unnamed protein product
Gene Aliases: 5330403K09; KIAA0226; mKIAA0226; RUBCN; RUBICON; SCAR15
UniProt ID: (Mouse) Q80U62, (Human) Q92622
Entrez Gene ID: (Mouse) 100502698, (Rat) 303885, (Human) 9711
biological_process
negative regulation of autophagy
negative regulation of endocytosis
endocytosis
autophagy
negative regulation of phosphatidylinositol 3-kinase activity
negative regulation of autophagosome maturation
immune system process
phagocytosis
negative regulation of protein kinase B signaling
multivesicular body sorting pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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