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Rubicon Polyclonal Antibody
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FIGURE: 1 / 3
Rubicon Antibody (600-401-EC8) in ICC/IF
Immunofluorescence Microscopy of Rabbit anti-RUBICON antibody. Tissue: human kidney. Primary antibody: RUBICON antibody at 20 µg/mL. Secondary antibody: Fluorescein rabbit secondary antibody at 1:20,000. Localization: RUBICON is located on the endosome and lysosome. Staining: RUBICON as red fluorescent signal.
Product Details
600-401-EC8
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Rubicon antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid peptide near the N-terminus of human Rubicon.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation. Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex. Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Baron; Beclin-1 associated RUN domain containing protein; Rubicon; RUN and cysteine rich domain containing beclin 1 interacting protein; RUN domain and cysteine-rich domain containing, Beclin 1-interacting protein; run domain Beclin-1 interacting and cystein-rich containing protein; run domain Beclin-1 interacting and cysteine-rich containing protein; Run domain Beclin-1-interacting and cysteine-rich domain-containing protein; rundataxin; similar to mKIAA0226 protein; unnamed protein product
Gene Aliases: 5330403K09; KIAA0226; mKIAA0226; RUBCN; RUBICON; SCAR15
UniProt ID: (Mouse) Q80U62, (Human) Q92622
Entrez Gene ID: (Mouse) 100502698, (Rat) 303885, (Human) 9711
biological_process
negative regulation of autophagy
negative regulation of endocytosis
endocytosis
autophagy
negative regulation of phosphatidylinositol 3-kinase activity
negative regulation of autophagosome maturation
immune system process
phagocytosis
negative regulation of protein kinase B signaling
multivesicular body sorting pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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