Western blot analysis of Rubicon using A) 30 ug 293T whole cell extract and B) 30 whole cell extract of human Rubicon-transfected 293T cells. Samples were loaded onto a 5% SDS-PAGE gel and probed with a Rubicon polyclonal antibody (Product # PA5-35969) at a dilution of 1:20000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 677 and 972 of Rubicon|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Negatively regulates endocytic trafficking. Impairs the autophagosome maturation process. May inhibit PIK3C3 activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.