Invitrogen

RyR1 Polyclonal Antibody

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RyR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RyR1 in paraffin-embedded rat skeletal muscle tissue sections using RyR1 Antibody (Product # PA5-111900) at a dilution of 1:100. RyR1 is expressed in fibers of striate muscle. Note that smooth muscle in the arterial walls is negative as well as the surrounding connective tissue. Hematoxilin is used as the counterstain.

RyR1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry analysis of RyR1 in frozen mouse cerebellum tissue sections using RyR1 Antibody (Product # PA5-111900) at a dilution of 1:100, (green). A) The highest expression of RyR1 is in the molecular layer (asterisk). B) In the same section, there is staining for parvalbumin (red), a marker of Purkinje cells. C) Merged image of panels A and B demonstrates that RyR1 is localized surrounding the dendritic tree of Purkinje cells. DAPI is used as the counterstain.

RyR1 Antibody in Western Blot (WB) — Western Blot analysis of RyR1 was performed in rat skeletal muscle lysates. Lane 1: RyR1 Antibody (Product # PA5-111900) at a dilution of 1:200. Lane 2: RyR1 Antibody preincubated with the negative control antigen.

Product Details

PA5-111900

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100	-
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide corresponding to amino acid residues 1371-1386 of human RyR1 (Intracellular, N-terminus) View antigen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.8 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_2857308

Product Specific Information

Reconstitute antibody with 50 µL of double distilled water (DDW). The reconstituted solution can be stored at 4 degrees C for up to 1 week. For longer periods, small aliquots should be stored at -20 degrees C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Peptide sequence is: (C)RAENEKD ATTEKNKKR

Homology: Rat, mouse amino acid residues are identical.

Target Information

Ryanodine receptors have been shown to play critical roles in the intracellular Ca2+ signaling occurring during cell activation in muscle cells and non-muscled cells. Ryanodine receptor, ~500 kDa, can be isolated from a variety of cell types such as lymphocytes, macrophages, granulocytes, fibroblasts, epithelial, endothelial cells, skeletal muscles, cardiac muscle and brain tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: calcium release channel; central core disease of muscle; protein phosphatase 1, regulatory subunit 137; Ryanodine receptor 1; ryanodine receptor 1 (skeletal); ryanodine receptor 1, skeletal muscle; ryanodine receptor 1-like, skeletal muscle; RYR-1; sarcoplasmic reticulum calcium release channel; Skeletal muscle calcium release channel; Skeletal muscle ryanodine receptor; Skeletal muscle-type ryanodine receptor; Type 1 ryanodine receptor; type 1-like ryanodine receptor

Gene Aliases: AI528790; CCO; MHS; MHS1; PPP1R137; RYDR; RYR; RYR-1; RYR1; Ryr1l; SKRR

UniProt ID: (Human) P21817, (Mouse) E9PZQ0

Entrez Gene ID: (Human) 6261, (Mouse) 20190, (Rat) 114207

Molecular Function: ligand-gated ion channel scaffold/adaptor protein