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S100A10 Polyclonal Antibody

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S100A10 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 11250-1-AP (S100A10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

S100A10 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 11250-1-AP (S100A10 Antibody) at dilution of 1:400 (under 40x lens).

S100A10 Antibody in Western Blot (WB) — Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 11250-1-AP (S100A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

S100A10 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug S100A10 antibody (11250-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

11250-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 3 publications 3 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	Assay-dependent	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	S100A10 Fusion Protein Ag1779 (1-97 aa encoded by BC015973)
Conjugate	Unconjugated
Form	Liquid
Concentration	0.29 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MPSQMEHAM ETMMFTFHKF AGDKGYLTKE DLRVLMEKEF PGFLENQKDP LAVDKIMKDL DQCRDGKVGF QSFFSLIAGL TIACNDYFVV HMKQKGKK (1-97 aa encoded by BC015973)

Target Information

The S-100 family of calcium-activated proteins interact with a range of target proteins to modulate biological signaling pathways. Numerous cancer cell lines overexpress the plasminogen receptor S-100A10 on the extracellular cell surface, where it forms a heterotetrameric complex with Annexin II, though this association is not required for plasma membrane localization or binding and activation of plasminogen. Additionally, S-100A10 acts as a cellular chaperone for hepatitis B (Hep B) virus polymerase. Hep B virus polymerase normally localizes to the cytoplasm only, though in the presence of S-100A10 a portion relocates to the nucleus, implying a role for S-100A10 and intracellular calcium in the process of viral replication.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

S100A10, a novel biomarker in pancreatic ductal adenocarcinoma.

Molecular oncology

Bydoun M,Sterea A,Liptay H,Uzans A,Huang WY,Rodrigues GJ,Weaver ICG,Gu H,Waisman DM

Thu Nov 01 00:00:00 EDT 2018

S100A10, a novel biomarker in pancreatic ductal adenocarcinoma.

Molecular oncology

Bydoun M,Sterea A,Liptay H,Uzans A,Huang WY,Rodrigues GJ,Weaver ICG,Gu H,Waisman DM

Thu Nov 01 00:00:00 EDT 2018

Probable involvement of p11 with interferon alpha induced depression.

Scientific reports

Guo J,Zhang W,Zhang L,Ding H,Zhang J,Song C,Zhang Y,Xia N,Li M,Liang Y,Hu X,Luan H,Wang H

Fri Jan 29 00:00:00 EST 2016

Probable involvement of p11 with interferon alpha induced depression.

Scientific reports

Guo J,Zhang W,Zhang L,Ding H,Zhang J,Song C,Zhang Y,Xia N,Li M,Liang Y,Hu X,Luan H,Wang H

Fri Jan 29 00:00:00 EST 2016

Label-free quantification proteomics reveals novel calcium binding proteins in matrix vesicles isolated from mineralizing Saos-2 cells.

Bioscience trends

Zhou X,Cui Y,Luan J,Zhou X,Zhang G,Zhang X,Han J

Sat Jun 01 00:00:00 EDT 2013

Feedback regulation of endothelial cell surface plasmin generation by PKC-dependent phosphorylation of annexin A2.

The Journal of biological chemistry

He KL,Sui G,Xiong H,Broekman MJ,Huang B,Marcus AJ,Hajjar KA

Fri Apr 29 00:00:00 EDT 2011

Feedback regulation of endothelial cell surface plasmin generation by PKC-dependent phosphorylation of annexin A2.

The Journal of biological chemistry

He KL,Sui G,Xiong H,Broekman MJ,Huang B,Marcus AJ,Hajjar KA

Fri Apr 29 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: annexin II ligand; annexin II ligand, calpactin I, light polypeptide; annexin II tetramer (AIIt) p11 subunit; calcium binding protein A11 (calgizzarin); Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; MGC111133; MGC133268; Nerve growth factor-induced protein 42C; OTTHUMP00000015270; P PRSS26; p10 protein; p11; p11 subunit; Protein S100 A10; Protein S100-A10; S-100 related protein, clone 42C; S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)); S100 calcium binding protein A10 (calgizzarin); S100 calcium-binding protein A10; S100 calcium-binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11))

Gene Aliases: 42C; AA409961; AL024248; ANX2L; ANX2LG; Ca[1]; CAL12; CAL1L; CLP11; GP11; p10; P11; S100A10

UniProt ID: (Human) P60903, (Rat) P05943, (Mouse) P08207

Entrez Gene ID: (Human) 6281, (Rat) 81778, (Mouse) 20194

Molecular Function: calmodulin-related

Function(s)

membrane raft assembly membrane budding positive regulation of GTPase activity positive regulation of binding protein heterotetramerization positive regulation of stress fiber assembly positive regulation of focal adhesion assembly establishment of protein localization to plasma membrane positive regulation of substrate adhesion-dependent cell spreading regulation of cell growth response to antidepressant regulation of cell differentiation cellular response to acid chemical

Process(es)

calcium ion binding protein binding lipid binding protein homodimerization activity ion channel binding

S100A10 Polyclonal Antibody

Product Details

11250-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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