Invitrogen

S100A10 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5 Published Figures

14 References

View all (24) S100A10 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

S100A10 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 19

S100A10 Antibody (PA5-95505) in ICC/IF

Immunofluorescence analysis of S100A10 in A431 cell. Antigen retrieval was performed on the tissue using IHC enzyme antigen retrieval reagent and blocked with 10% goat serum. Samples were incubated with S100A10 polyclonal antibody (Product # PA5-95505) at a 2 µg/1x10^6 cells dilution, followed by 488 conjugated goat anti-rabbit IgG using a 1:100 dilution and DAPI.

S100A10 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

S100A10 Antibody in Flow Cytometry (Flow)

S100A10 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

S100A10 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

S100A10 Antibody in Immunohistochemistry (IHC)

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Product Details

PA5-95505

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 3 publications 3 publications

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

ELISA (ELISA)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human S100A10 recombinant protein (Position: Q4-K94).

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2807307

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

The S-100 family of calcium-activated proteins interact with a range of target proteins to modulate biological signaling pathways. Numerous cancer cell lines overexpress the plasminogen receptor S-100A10 on the extracellular cell surface, where it forms a heterotetrameric complex with Annexin II, though this association is not required for plasma membrane localization or binding and activation of plasminogen. Additionally, S-100A10 acts as a cellular chaperone for hepatitis B (Hep B) virus polymerase. Hep B virus polymerase normally localizes to the cytoplasm only, though in the presence of S-100A10 a portion relocates to the nucleus, implying a role for S-100A10 and intracellular calcium in the process of viral replication.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chronic spinal cord compression associated with intervertebral disc degeneration in SPARC-null mice.

Neural regeneration research

Li ZY,Zhou AF,Li G,Zhou LY,Pu PM,Zhu K,Zheng Z,Wang YJ,Liang QQ,Yao M,Cui XJ

Published figure using S100A10 polyclonal antibody (Product # PA5-95505) in Immunohistochemistry

Wed Mar 01 00:00:00 EST 2023

Chronic spinal cord compression associated with intervertebral disc degeneration in SPARC-null mice.

Neural regeneration research

Li ZY,Zhou AF,Li G,Zhou LY,Pu PM,Zhu K,Zheng Z,Wang YJ,Liang QQ,Yao M,Cui XJ

Published figure using S100A10 polyclonal antibody (Product # PA5-95505) in Immunohistochemistry

Wed Mar 01 00:00:00 EST 2023

Chronic spinal cord compression associated with intervertebral disc degeneration in SPARC-null mice.

Neural regeneration research

Li ZY,Zhou AF,Li G,Zhou LY,Pu PM,Zhu K,Zheng Z,Wang YJ,Liang QQ,Yao M,Cui XJ

Published figure using S100A10 polyclonal antibody (Product # PA5-95505) in Immunohistochemistry

Wed Mar 01 00:00:00 EST 2023

The critical role of KLF4 in regulating the activation of A1/A2 reactive astrocytes following ischemic stroke.

Journal of neuroinflammation

Wang C,Li L

PA5-95505 was used in Immunohistochemistry-immunofluorescence to demonstrate that astrocytic KLF4 expression and complement C3-positive A1 and S100 calcium binding protein A10 (S100A10)-positive A2 astrocytes were induced in the ischemic penumbra following focal cerebral ischemia, and the time course of upregulation of astrocytic KLF4 correlated closely with the activation of A2 astrocytes.

Thu Feb 23 00:00:00 EST 2023

Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia.

EBioMedicine

Xu T,Li X,Guo Y,Uhlin E,Holmberg L,Mitra S,Winn D,Falk A,Sundström E

Published figure using S100A10 polyclonal antibody (Product # PA5-95505) in Immunohistochemistry

Tue Mar 01 00:00:00 EST 2022

Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia.

EBioMedicine

Xu T,Li X,Guo Y,Uhlin E,Holmberg L,Mitra S,Winn D,Falk A,Sundström E

Published figure using S100A10 polyclonal antibody (Product # PA5-95505) in Immunohistochemistry

Tue Mar 01 00:00:00 EST 2022

Mesenchymal Stem Cells Improve Cognitive Impairment and Reduce Aβ Deposition via Promoting AQP4 Polarity and Relieving Neuroinflammation in Rats With Chronic Hypertension-Induced Cerebral Small-Vessel Disease.

Frontiers in aging neuroscience

Liu XL,Ouyang FB,Hu LT,Sun P,Yang J,Sun YJ,Liao MS,Lan LF,Pei Z,Fan YH

PA5-95505 was used in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to disclose the roles of aquaporin 4 (AQP4) and neuroinflammation in CSVD, which were the key factors for Aβ clearance and production, respectively, and the effect of mesenchymal stem cells (MSCs) on Aβ deposition and these two factors.

Sat Jul 16 00:00:00 EDT 2022

Sequential progenitor states mark the generation of pancreatic endocrine lineages in mice and humans.

Cell research

Yu XX,Qiu WL,Yang L,Wang YC,He MY,Wang D,Zhang Y,Li LC,Zhang J,Wang Y,Xu CR

PA5-95505 was used in Immunohistochemistry-immunofluorescence to support a model in which sequential transient progenitor cell states determine the differentiation of multiple cell lineages and provide a blueprint for directing the generation of pancreatic islets in vitro.

Sun Aug 01 00:00:00 EDT 2021

AIM2 inflammasome mediates hallmark neuropathological alterations and cognitive impairment in a mouse model of vascular dementia.

Molecular psychiatry

Poh L,Fann DY,Wong P,Lim HM,Foo SL,Kang SW,Rajeev V,Selvaraji S,Iyer VR,Parathy N,Khan MB,Hess DC,Jo DG,Drummond GR,Sobey CG,Lai MKP,Chen CL,Lim LHK,Arumugam TV

PA5-95505 was used in Immunohistochemistry (Paraffin) to investigate its effect on the innate immune response-particularly the inflammasome signaling pathway using a bilateral common carotid artery stenosis (BCAS) mouse model of vascular dementia (VaD).

Sun Aug 01 00:00:00 EDT 2021

Melatonin Ameliorates Axonal Hypomyelination of Periventricular White Matter by Transforming A1 to A2 Astrocyte via JAK2/STAT3 Pathway in Septic Neonatal Rats.

Journal of inflammation research

Jiang S,Wang H,Zhou Q,Li Q,Liu N,Li Z,Chen C,Deng Y