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Invitrogen
S100A8 Polyclonal Antibody
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FIGURE: 1 / 11
S100A8 Antibody (PA5-79948) in ICC/IF
Immunocytochemistry analysis of S100A8 using anti-S100A8 antibody (Product # PA5-79948). S100A8 was detected in a section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-S100A8 antibody (Product # PA5-79948) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visu... View More
Product Details
PA5-79948
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived mouse S100A8 recombinant protein (Position: P2-E89).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.01mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat spleen tissue, rat thymus tissue, mouse spleen tissue, mouse thymus tissue. IHC: rat spleen tissue, mouse spleen tissue. ICC/IF: NIH3T3 cell. Flow: HEPA 1-6 cell.
Target Information
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Calgranulin-A; Chemotactic cytokine CP-10; chemotactic S100 protein; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; Pro-inflammatory S100 cytokine; Protein S100-A8; S100 A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A)
Gene Aliases: 60B8Ag; AI323541; B8Ag; Caga; CFAg; CP-10; MRP8; p8; S100a8
UniProt ID: (Rat) P50115, (Mouse) P27005
Entrez Gene ID: (Rat) 116547, (Mouse) 20201
leukocyte migration involved in inflammatory response
acute inflammatory response
chronic inflammatory response
positive regulation of peptide secretion
autophagy
activation of cysteine-type endopeptidase activity involved in apoptotic process
inflammatory response
response to zinc ion
neutrophil chemotaxis
response to lipopolysaccharide
wound healing
innate immune response
response to ethanol
positive regulation of inflammatory response
neutrophil aggregation
positive regulation of intrinsic apoptotic signaling pathway
immune system process
apoptotic process
chemotaxis
peptidyl-cysteine S-nitrosylation
regulation of inflammatory response
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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