Proteintech

S100A9 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (16)

S100A9 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

S100A9 Antibody (26992-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 26992-1-AP (S100A9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

S100A9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

S100A9 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

26992-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 4 publications 4 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

S100A9 Fusion Protein Ag25764 (25-114 aa encoded by BC047681)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KLGHPDTLN QGEFKELVRK DLQNFLKKEN KNEKVIEHIM EDLDTNADKQ LSFEEFIMLM ARLTWASHEK MHEGDEGPGH HHKPGLGEGT P (25-114 aa encoded by BC047681)

Target Information

S100A9 (MRP-14) is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Senescent immune cells accumulation promotes brown adipose tissue dysfunction during aging.

Nature communications

Feng X,Wang L,Zhou R,Zhou R,Chen L,Peng H,Huang Y,Guo Q,Luo X,Zhou H

Fri Jun 02 00:00:00 EDT 2023

Senescent immune cells accumulation promotes brown adipose tissue dysfunction during aging.

Nature communications

Feng X,Wang L,Zhou R,Zhou R,Chen L,Peng H,Huang Y,Guo Q,Luo X,Zhou H

Fri Jun 02 00:00:00 EDT 2023

Identification of S100A9 as a Potential Inflammation-Related Biomarker for Radiation-Induced Lung Injury.

Journal of clinical medicine

Liu Y,Wu M,Guo J,Tang Y,Jiang H,Yang B,Wu M,Huang J

Tue Jan 17 00:00:00 EST 2023

Hemin protects UVB-induced skin damage through inhibiting keratinocytes apoptosis and reducing neutrophil infiltration.

Journal of photochemistry and photobiology. B, Biology

Bai F,Fan C,Lin X,Wang HY,Wu B,Feng CL,Zhou R,Wu YW,Tang W

Sun Jan 01 00:00:00 EST 2023

Integrated MicroRNA and Secretome Analysis of Human Endometrial Organoids Reveal the miR-3194-5p/Aquaporin/S100A9 Module in Regulating Trophoblast Functions.

Molecular & cellular proteomics : MCP

Dong Y,Li J,Cao D,Zhong J,Liu X,Duan YG,Lee KF,Yeung WSB,Lee CL,Chiu PCN

Sat Apr 01 00:00:00 EDT 2023

Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells.

Theranostics

Liu G,Wang Q,Deng L,Huang X,Yang G,Cheng Q,Guo T,Guo L,Niu C,Yang X,Dong J,Zhang J

Thu Apr 21 00:00:00 EDT 2022

Hepatic RACK1 deficiency protects against fulminant hepatitis through myeloid-derived suppressor cells.

Theranostics

Liu G,Wang Q,Deng L,Huang X,Yang G,Cheng Q,Guo T,Guo L,Niu C,Yang X,Dong J,Zhang J

Thu Apr 21 00:00:00 EDT 2022

The role of S100A9 in the interaction between pancreatic ductal adenocarcinoma cells and stromal cells.

Cancer immunology, immunotherapy : CII

Kung PJ,Lai TY,Cao J,Hsu LC,Chiang TC,Ou-Yang P,Tsai CY,Tsai YF,Lin CW,Chen CC,Tsai MK,Tien YW,Lee CY

Tue Mar 01 00:00:00 EST 2022

Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.

Cell research

Zhang S,Huang W,Ren L,Ju X,Gong M,Rao J,Sun L,Li P,Ding Q,Wang J,Zhang QC

Sat Jan 01 00:00:00 EST 2022

The Infiltration of Neutrophil Granulocytes Due to Loss of PTEN Was Associated with Poor Response to Immunotherapy in Renal Cell Carcinoma.

Journal of inflammation research

Wu F,Chen J,Yao K,Fan D,Wang M,Liu Y,Xin S,Sun Z,Li S,Sun Y,Liu Q

Wed Dec 21 00:00:00 EST 2022

Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro.

Stem cells international

Li JQ,Jiang HJ,Su XY,Feng L,Zhan NZ,Li SS,Chen ZJ,Chang BH,Cheng PZ,Yang L,Pei GX

Tue Jan 25 00:00:00 EST 2022

Shuangxinfang Prevents S100A9-Induced Macrophage/Microglial Inflammation to Improve Cardiac Function and Depression-Like Behavior in Rats After Acute Myocardial Infarction.

Frontiers in pharmacology

Sun Y,Wang Z,Hou J,Shi J,Tang Z,Wang C,Zhao H

Sat Jul 16 00:00:00 EDT 2022

MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.

Genomics

Shi Y,He W,Zhong M,Yu M

Thu Jul 01 00:00:00 EDT 2021

Bmal1 Regulates Macrophage Polarize Through Glycolytic Pathway in Alcoholic Liver Disease.

Frontiers in pharmacology

Zhou Y,Wu M,Xu L,Cheng J,Shen J,Yang T,Zhang L

Fri Apr 02 00:00:00 EDT 2021

Keratin 1 attenuates hypoxic pulmonary artery hypertension by suppressing pulmonary artery media smooth muscle expansion.

Acta physiologica (Oxford, England)

Zhang L,Zeng XX,Li YM,Chen SK,Tang LY,Wang N,Yang X,Lin MJ

Mon Feb 01 00:00:00 EST 2021

The "Matrisome" reveals the characterization of skin keloid microenvironment.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang S,Liu B,Wang W,Lv L,Gao D,Chai M,Li M,Wu Z,Zhu Y,Ma J,Leng L

Thu Apr 01 00:00:00 EDT 2021

The "Matrisome" reveals the characterization of skin keloid microenvironment.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhang S,Liu B,Wang W,Lv L,Gao D,Chai M,Li M,Wu Z,Zhu Y,Ma J,Leng L

Thu Apr 01 00:00:00 EDT 2021

Psycho-cardiology therapeutic effects of Shuangxinfang in rats with depression-behavior post acute myocardial infarction: Focus on protein S100A9 from proteomics.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Sun Y,Wang Z,Wang C,Tang Z,Zhao H

Wed Dec 01 00:00:00 EST 2021

Gut microbiota from patients with arteriosclerotic CSVD induces higher IL-17A production in neutrophils via activating RORγt.

Science advances

Cai W,Chen X,Men X,Ruan H,Hu M,Liu S,Lu T,Liao J,Zhang B,Lu D,Huang Y,Fan P,Rao J,Lei C,Wang J,Ma X,Zhu Q,Li L,Zhu X,Hou Y,Li S,Dong Q,Tian Q,Ai L,Luo W,Zuo M,Shen L,Xie C,Song H,Xu G,Zheng K,Zhang Z,Lu Y,Qiu W,Chen T,Xiang AP,Lu Z

Fri Jan 01 00:00:00 EST 2021

Ovarian cancer modulates the immunosuppressive function of CD11b⁺Gr1⁺ myeloid cells via glutamine metabolism.

Molecular metabolism

Udumula MP,Sakr S,Dar S,Alvero AB,Ali-Fehmi R,Abdulfatah E,Li J,Jiang J,Tang A,Buekers T,Morris R,Munkarah A,Giri S,Rattan R

Mon Nov 01 00:00:00 EDT 2021

Triptolide analog LLDT-8 ameliorates psoriasis-like dermatitis in BALB/c mice via suppressing the IL-36α signaling pathway.

Pharmacological research

Qi Q,Li Q,Zhu H,Lu H,Yang X,Wu Y,Feng C,Fan C,Li H,Wu B,Gao Y,Zhang Z,Zhou H,Zuo J,Tang W

Thu Jul 01 00:00:00 EDT 2021

Pancreatic ductal deletion of S100A9 alleviates acute pancreatitis by targeting VNN1-mediated ROS release to inhibit NLRP3 activation.

Theranostics

Xiang H,Guo F,Tao X,Zhou Q,Xia S,Deng D,Li L,Shang D

Mon Jul 26 00:00:00 EDT 2021

Identification of lysine acetylome in cervical cancer by label-free quantitative proteomics.

Cancer cell international

Zhang L,Wang W,Zhang S,Wang Y,Guo W,Liu Y,Wang Y,Zhang Y

Thu Apr 14 00:00:00 EDT 2022

Identification of differentially expressed proteins associated with recurrence in ovarian endometriotic cysts.

Systems biology in reproductive medicine

Zhu G,Kong J,Fu X,Liu F,Huang H,Hong L,Wang K

Sat Feb 01 00:00:00 EST 2020

Global Proteome and Phosphoproteome Characterization of Sepsis-induced Kidney Injury.

Molecular & cellular proteomics : MCP

Lin YH,Platt MP,Fu H,Gui Y,Wang Y,Gonzalez-Juarbe N,Zhou D,Yu Y

Tue Dec 01 00:00:00 EST 2020

Proteomic analysis of human periodontal ligament cells under hypoxia.

Proteome science

Li Q,Luo T,Lu W,Yi X,Zhao Z,Liu J

Sun Apr 10 00:00:00 EDT 2022

Comparative proteomic profiling of human dental pulp stem cells and periodontal ligament stem cells under in vitro osteogenic induction.

Archives of oral biology

Wang H,Ma D,Zhang X,Xu S,Ning T,Wu B

Tue May 01 00:00:00 EDT 2018

Comparative proteomic profiling of human dental pulp stem cells and periodontal ligament stem cells under in vitro osteogenic induction.

Archives of oral biology

Wang H,Ma D,Zhang X,Xu S,Ning T,Wu B

Tue May 01 00:00:00 EDT 2018

Quantitative proteomics analysis of differentially expressed proteins in ruptured and unruptured cerebral aneurysms by iTRAQ.

Journal of proteomics

Jiang P,Wu J,Chen X,Ning B,Liu Q,Li Z,Li M,Yang F,Cao Y,Wang R,Wang S

Sat Jun 30 00:00:00 EDT 2018

Pancreatic cancer-derived exosomes promote tumor metastasis and liver pre-metastatic niche formation.

Oncotarget

Yu Z,Zhao S,Ren L,Wang L,Chen Z,Hoffman RM,Zhou J

Fri Sep 08 00:00:00 EDT 2017

Pancreatic cancer-derived exosomes promote tumor metastasis and liver pre-metastatic niche formation.

Oncotarget

Yu Z,Zhao S,Ren L,Wang L,Chen Z,Hoffman RM,Zhou J

Fri Sep 08 00:00:00 EDT 2017

Pancreatic cancer-derived exosomes promote tumor metastasis and liver pre-metastatic niche formation.

Oncotarget

Yu Z,Zhao S,Ren L,Wang L,Chen Z,Hoffman RM,Zhou J

Fri Sep 08 00:00:00 EDT 2017

Pancreatic cancer-derived exosomes promote tumor metastasis and liver pre-metastatic niche formation.

Oncotarget

Yu Z,Zhao S,Ren L,Wang L,Chen Z,Hoffman RM,Zhou J

Fri Sep 08 00:00:00 EDT 2017

Proteomic analyses of Urine Exosomes reveal New Biomarkers of Diabetes in Pregnancy.

Madridge journal of diabetes

Ramachandrarao SP,Hamlin AA,Awdishu L,Overcash R,Zhou M,Proudfoot J,Ishaya M,Aghania E,Madrigal A,Kokoy-Mondragon C,Kao K,Khoshaba R,Bounkhoun A,Ghassemian M,Tarsa M,Naviaux RK

Sun Apr 10 00:00:00 EDT 2022

Bioinformatics

Protein Aliases: BM-009; calgranulin B; Calgranulin-B; Calprotectin L1H subunit; DKFZp686B04128; Leukocyte L1 complex heavy chain; Macrophage; Migration inhibitory factor-related protein 14; Monocyte; MRP-14; MRP15; MRP16; Protein S100-A9; S100 A9; S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B)

View more View less

Gene Aliases: 60B8AG; AW546964; BEE22; CAGB; CFAG; CGLB; GAGB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14; S100A9

View more View less

UniProt ID: (Human) P06702, (Mouse) P31725

View more View less

Entrez Gene ID: (Human) 6280, (Mouse) 20202

View more View less

Function(s)

signal transducer activity calcium ion binding protein binding microtubule binding zinc ion binding antioxidant activity Toll-like receptor 4 binding arachidonic acid binding RAGE receptor binding metal ion binding calmodulin-related

Process(es)

cytokine production leukocyte migration involved in inflammatory response positive regulation of peptide secretion regulation of translation autophagy apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response signal transduction cell-cell signaling astrocyte development positive regulation of blood coagulation positive regulation of cell growth neutrophil chemotaxis actin cytoskeleton reorganization sequestering of zinc ion chemokine production peptidyl-cysteine S-trans-nitrosylation defense response to bacterium innate immune response regulation of integrin biosynthetic process positive regulation of inflammatory response defense response to fungus positive regulation of NF-kappaB transcription factor activity regulation of cytoskeleton organization neutrophil aggregation cellular oxidant detoxification positive regulation of intrinsic apoptotic signaling pathway immune system process chemotaxis peptidyl-cysteine S-nitrosylation leukocyte chemotaxis regulation of inflammatory response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

S100A9 Polyclonal Antibody

S100A9 Antibody (26992-1-AP) in ICC/IF

Product Details

26992-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!