Invitrogen

S100A9 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

5 Published Figures

5 References

View all (56) S100A9 antibodies

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Datasheet

Protocols

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S100A9 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of S100A9 was performed using log phase THP-1 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with S100A9 Polyclonal Antibody (Product # PA1-46489) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to plasma membrane, nucleus and cytoplasm. Panel e shows Raji cells with no expression of S100A9. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

S100A9 Antibody in Western Blot (WB) — Western blot was performed using Anti-S100A9 Polyclonal Antibody (Product # PA1-46489) and a 13kDa band corresponding to S100A9 was observed in SK-BR-3, THP-1, Mouse Spleen and Rat Spleen but was absent in Raji, SH-SY5Y, Mouse Brain and Rat Brain which are reported to be negative. Expression of Calprotectin S100A9 was downregulated upon treatment of THP-1 with PMA/TPA [10.1073/pnas.0709958105]. Membrane enriched cell extracts (30 µg lysate) of SK-BR-3 (Lane 1), THP-1 (Lane 2), THP-1 treated with PMA/TPA (50ng/ml for 24 hours) (Lane 3), Raji (Lane 4), SH-SY5Y (Lane 5), Mouse Spleen (Lane 6), Rat Spleen (Lane 7), Mouse Brain (Lane 8) and Rat Brain (Lane 9) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

S100A9 Antibody in Immunohistochemistry (IHC) — Histological analysis of proteins involved in epidermal barrier function. Immunohistochemical staining (brown) of filaggrin, loricrin, Ki-67 and S100A9 in skin biopsies from patients with extrinsic atopic dermatitis ( AD ex), intrinsic AD ( AD in) and healthy controls ( NN ). (a) Filaggrin staining of skin samples from NN and patients with a detected mutation in FLG at the indicated loci: 2282del4, R2447x or R501x. (b) Loricrin staining of skin samples from NN and patients with a mutation in FLG . (c) Ki-67 staining of skin samples from NN and patients with extrinsic and intrinsic AD , respectively. (d) S100A9 staining of skin samples from NN and patients with extrinsic and intrinsic AD , respectively. Numbers correspond to the ID of the specific subjects as in Table S1. Scale bars = 250 mu m.

S100A9 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-S100A9 Polyclonal Antibody (Product # PA1-46489) shows increased expression of S100A9 in THP-1 cell line when compared to Raji. {RE}

Product Details

PA1-46489

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	Assay-Dependent	-
Immunocytochemistry (ICC/IF)	1:50-1:200	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Full length human S100A9 protein expressed in E. coli. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	whole serum
Contains	0.1% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10977234

Product Specific Information

Suggested positive control: antigen standard for S100A9 (transient overexpression lysate), HL-60 + DMSO.

Target Information

S100A9 (MRP-14) is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn.

Aging

Xu Z,Cheng C,Kong R,Liu Y,Wang S,Ma Y,Xing X

PA1-46489 was used in Immunocytochemistry to find that S100A8 and S100A9 were both upregulated in burn-wounded skins in vivo and thermal-stimulated epidermal keratinocytes in vitro, accompanied by increased levels of epithelial-mesenchymal transition.

Mon Jun 07 00:00:00 EDT 2021

Quantitative proteomic characterization of microvesicles/exosomes from the cerebrospinal fluid of patients with acute bilirubin encephalopathy.

Molecular medicine reports

Tan N,Hu S,Hu Z,Wu Z,Wang B

PA1-46489 was used in Western Blotting to identify differentially expressed proteins (DEPs) in microvesicles/exosomes (MV/E) isolated from the cerebrospinal fluid (CSF) of patients with ABE.

Sat Aug 01 00:00:00 EDT 2020

Quantitative proteomic characterization of microvesicles/exosomes from the cerebrospinal fluid of patients with acute bilirubin encephalopathy.

Molecular medicine reports

Tan N,Hu S,Hu Z,Wu Z,Wang B

PA1-46489 was used in Western Blotting to identify differentially expressed proteins (DEPs) in microvesicles/exosomes (MV/E) isolated from the cerebrospinal fluid (CSF) of patients with ABE.

Sat Aug 01 00:00:00 EDT 2020

Distinct molecular signatures of mild extrinsic and intrinsic atopic dermatitis.

Experimental dermatology

Martel BC,Litman T,Hald A,Norsgaard H,Lovato P,Dyring-Andersen B,Skov L,Thestrup-Pedersen K,Skov S,Skak K,Poulsen LK

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Immunohistochemistry

Wed Jun 01 00:00:00 EDT 2016

Analysis of protein expression in periodontal pocket tissue: a preliminary study.

Proteome science

Monari E,Cuoghi A,Bellei E,Bergamini S,Lucchi A,Tomasi A,Cortellini P,Zaffe D,Bertoldi C

PA1-46489 was used in western blot to determine the proteomic profile of periodontally-affected patients

Thu Dec 31 00:00:00 EST 2015

Analysis of protein expression in periodontal pocket tissue: a preliminary study.

Proteome science

Monari E,Cuoghi A,Bellei E,Bergamini S,Lucchi A,Tomasi A,Cortellini P,Zaffe D,Bertoldi C

PA1-46489 was used in western blot to determine the proteomic profile of periodontally-affected patients

Thu Dec 31 00:00:00 EST 2015

Bioinformatics

Protein Aliases: BM-009; calgranulin B; Calgranulin-B; Calprotectin L1H subunit; DKFZp686B04128; intracellular calcium-binding protein (MRP14); Leukocyte L1 complex heavy chain; Macrophage; Migration inhibitory factor-related protein 14; Monocyte; MRP-14; MRP15; MRP16; Myeloid-related protein 14; p14; Protein S100-A9; S100 A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B)

Gene Aliases: 60B8AG; AW546964; BEE22; CAGB; CFAG; CGLB; GAGB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14; S100A9

UniProt ID: (Human) P06702, (Mouse) P31725, (Rat) P50116

Entrez Gene ID: (Human) 6280, (Mouse) 20202, (Rat) 94195

Molecular Function: calmodulin-related