Invitrogen

S100A9 Polyclonal Antibody

S100A9 Antibody in Immunocytochemistry (ICC/IF)

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S100A9 Antibody (PA1-46489) in ICC/IF

Immunofluorescence analysis of S100A9 was performed using log phase THP-1 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with S100A9 Polyclonal Antibody (Product # PA1-46489) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at... View More

S100A9 Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-46489

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunocytochemistry (ICC/IF)

1:50-1:200

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human S100A9 protein expressed in E. coli.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10977234

Product Specific Information

Suggested positive control: antigen standard for S100A9 (transient overexpression lysate), HL-60 + DMSO.

Target Information

S100A9 (MRP-14) is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ApoE Isoforms Inhibit Amyloid Aggregation of Proinflammatory Protein S100A9.

International journal of molecular sciences

Ghosh S,Tamilselvi S,Williams C,Jayaweera SW,Iashchishyn IA,Šulskis D,Gilthorpe JD,Olofsson A,Smirnovas V,Svedružić ŽM,Morozova-Roche LA

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Western Blot

Fri Feb 09 00:00:00 UTC 2024

Quantitative proteomic landscape of unstable atherosclerosis identifies molecular signatures and therapeutic targets for plaque stabilization.

Communications biology

Chen YC,Smith M,Ying YL,Makridakis M,Noonan J,Kanellakis P,Rai A,Salim A,Murphy A,Bobik A,Vlahou A,Greening DW,Peter K

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Western Blot

Mon Mar 13 00:00:00 UTC 2023

Quantitative proteomic landscape of unstable atherosclerosis identifies molecular signatures and therapeutic targets for plaque stabilization.

Communications biology

Chen YC,Smith M,Ying YL,Makridakis M,Noonan J,Kanellakis P,Rai A,Salim A,Murphy A,Bobik A,Vlahou A,Greening DW,Peter K

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Western Blot

Mon Mar 13 00:00:00 UTC 2023

Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters.

The Journal of cell biology

Stempels FC,Jiang M,Warner HM,Moser ML,Janssens MH,Maassen S,Nelen IH,de Boer R,Jiemy WF,Knight D,Selley J,O'Cualain R,Baranov MV,Burgers TCQ,Sansevrino R,Milovanovic D,Heeringa P,Jones MC,Vlijm R,Ter Beest M,van den Bogaart G

PA1-46489 was used in Immunocytochemistry to propose that the ESCRT proteins are recruited to adhesion-induced membrane tears to induce extracellular shedding of the damaged membrane.

Mon Jul 03 00:00:00 UTC 2023

Rapamycin-filgrastim combination therapy ameliorates portal hypertension-induced splenomegaly: Role of β actin and S100A9 proteins modulation.

Iranian journal of basic medical sciences

Abdelrahman SA,Abdelfatah MM,Keshta AT

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Immunohistochemistry

Wed Jun 01 00:00:00 UTC 2022

Rapamycin-filgrastim combination therapy ameliorates portal hypertension-induced splenomegaly: Role of β actin and S100A9 proteins modulation.

Iranian journal of basic medical sciences

Abdelrahman SA,Abdelfatah MM,Keshta AT

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Immunohistochemistry

Wed Jun 01 00:00:00 UTC 2022

S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn.

Aging

Xu Z,Cheng C,Kong R,Liu Y,Wang S,Ma Y,Xing X

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Immunocytochemistry

Mon Jun 07 00:00:00 UTC 2021

S100A8 and S100A9, both transcriptionally regulated by PU.1, promote epithelial-mesenchymal transformation (EMT) and invasive growth of dermal keratinocytes during scar formation post burn.

Aging

Xu Z,Cheng C,Kong R,Liu Y,Wang S,Ma Y,Xing X

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Immunocytochemistry

Mon Jun 07 00:00:00 UTC 2021

Quantitative proteomic characterization of microvesicles/exosomes from the cerebrospinal fluid of patients with acute bilirubin encephalopathy.

Molecular medicine reports

Tan N,Hu S,Hu Z,Wu Z,Wang B

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Western Blot

Sat Aug 01 00:00:00 UTC 2020

Quantitative proteomic characterization of microvesicles/exosomes from the cerebrospinal fluid of patients with acute bilirubin encephalopathy.

Molecular medicine reports

Tan N,Hu S,Hu Z,Wu Z,Wang B

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Western Blot

Sat Aug 01 00:00:00 UTC 2020

Distinct molecular signatures of mild extrinsic and intrinsic atopic dermatitis.

Experimental dermatology

Martel BC,Litman T,Hald A,Norsgaard H,Lovato P,Dyring-Andersen B,Skov L,Thestrup-Pedersen K,Skov S,Skak K,Poulsen LK

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Immunohistochemistry

Wed Jun 01 00:00:00 UTC 2016

Distinct molecular signatures of mild extrinsic and intrinsic atopic dermatitis.

Experimental dermatology

Martel BC,Litman T,Hald A,Norsgaard H,Lovato P,Dyring-Andersen B,Skov L,Thestrup-Pedersen K,Skov S,Skak K,Poulsen LK

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Immunohistochemistry

Wed Jun 01 00:00:00 UTC 2016

Analysis of protein expression in periodontal pocket tissue: a preliminary study.

Proteome science

Monari E,Cuoghi A,Bellei E,Bergamini S,Lucchi A,Tomasi A,Cortellini P,Zaffe D,Bertoldi C

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Western Blot

Thu Dec 31 00:00:00 UTC 2015

Analysis of protein expression in periodontal pocket tissue: a preliminary study.

Proteome science

Monari E,Cuoghi A,Bellei E,Bergamini S,Lucchi A,Tomasi A,Cortellini P,Zaffe D,Bertoldi C

Published figure using S100A9 polyclonal antibody (Product # PA1-46489) in Western Blot

Thu Dec 31 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: BM-009; calgranulin B; Calgranulin-B; Calprotectin L1H subunit; DKFZp686B04128; intracellular calcium-binding protein (MRP14); Leukocyte L1 complex heavy chain; Macrophage; Migration inhibitory factor-related protein 14; Monocyte; MRP-14; MRP15; MRP16; Myeloid-related protein 14; p14; Protein S100-A9; S100 A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B)

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Gene Aliases: 60B8AG; AW546964; BEE22; CAGB; CFAG; CGLB; GAGB; L1AG; LIAG; MAC387; MIF; MRP14; NIF; P14; S100A9

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UniProt ID: (Human) P06702, (Mouse) P31725, (Rat) P50116

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Entrez Gene ID: (Human) 6280, (Mouse) 20202, (Rat) 94195

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Function(s)

signal transducer activity calcium ion binding protein binding microtubule binding zinc ion binding antioxidant activity Toll-like receptor 4 binding arachidonic acid binding RAGE receptor binding metal ion binding molecular_function calmodulin-related

Process(es)

cytokine production leukocyte migration involved in inflammatory response positive regulation of peptide secretion regulation of translation autophagy apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response signal transduction cell-cell signaling astrocyte development positive regulation of blood coagulation positive regulation of cell growth neutrophil chemotaxis actin cytoskeleton reorganization sequestering of zinc ion chemokine production peptidyl-cysteine S-trans-nitrosylation defense response to bacterium innate immune response regulation of integrin biosynthetic process positive regulation of inflammatory response defense response to fungus positive regulation of NF-kappaB transcription factor activity regulation of cytoskeleton organization neutrophil aggregation cellular oxidant detoxification positive regulation of intrinsic apoptotic signaling pathway immune system process chemotaxis peptidyl-cysteine S-nitrosylation leukocyte chemotaxis regulation of inflammatory response chronic inflammatory response response to zinc ion response to lipopolysaccharide response to ethanol

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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S100A9 Polyclonal Antibody

S100A9 Antibody (PA1-46489) in ICC/IF

Product Details

PA1-46489

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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