Invitrogen

S1P1 Polyclonal Antibody

14 Published Figures

14 References

View all (32) S1P1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Published Figures (14)

S1P1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 18

S1P1 Antibody (PA1-1040) in ICC/IF

Immunofluorescence analysis of EDG1 was performed using 70% confluent log phase Neuro-2a cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EDG1 Rabbit Polyclonal Antibody (Product # PA1-1040) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

S1P1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

S1P1 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

S1P1 Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-1040

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

View 8 publications 8 publications

Immunohistochemistry (IHC)

Assay-dependent

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2184729

Product Specific Information

PA1-1040 detects S1P1 protein in transfected human and mouse cell samples. This antibody shows no cross-reactivity to S1P3.

PA1-1040 has successfully been used in immuno-fluorescence, immunohistochemistry, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. Immunofluorescence data demonstrates that S1P1 is localized to the plasma membrane after staining with PA1-1040.

The PA1-1040 immunogen is a synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1. This sequence is completely conserved in rat and 92% conserved in human S1P1. This peptide (Cat. # PEP-220) is available for use in neutralization and control experiments.

Target Information

S1P1 is a G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that is coupled to the G(i) subclass of heteromeric G proteins. S1P1 signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. It plays an important role in cell migration via its role in the reorganization of the actin cytoskeleton and formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. S1P1 is required for normal chemotaxis toward sphingosine 1-phosphate, normal embryonic heart development, and normal cardiac morphogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chlorogenic acid attenuates cardiac hypertrophy via up-regulating Sphingosine-1-phosphate receptor1 to inhibit endoplasmic reticulum stress.

ESC heart failure

Ping P,Yang T,Ning C,Zhao Q,Zhao Y,Yang T,Gao Z,Fu S

Published figure using S1P1 polyclonal antibody (Product # PA1-1040) in Western Blot

Sat Jun 01 00:00:00 UTC 2024

Density of Sphingosine-1-Phosphate Receptors Is Altered in Cortical Nerve-Terminals of Insulin-Resistant Goto-Kakizaki Rats and Diet-Induced Obese Mice.

Neurochemical research

Skoug C,Erdogan H,Vanherle L,Vieira JPP,Matthes F,Eliasson L,Meissner A,Duarte JMN

Published figure using S1P1 polyclonal antibody (Product # PA1-1040) in Western Blot

Thu Feb 01 00:00:00 UTC 2024

Sphingosine-1-Phosphate Receptor 3 Induces Endothelial Barrier Loss via ADAM10-Mediated Vascular Endothelial-Cadherin Cleavage.

International journal of molecular sciences

Wu J,Liang Y,Fu P,Feng A,Lu Q,Unwalla HJ,Marciano DP,Black SM,Wang T

Published figure using S1P1 polyclonal antibody (Product # PA1-1040) in Western Blot

Wed Nov 08 00:00:00 UTC 2023

Dysregulation of sphingosine-1-phosphate (S1P) and S1P receptor 1 signaling in the 5xFAD mouse model of Alzheimer's disease.

Brain research

Jung Y,Lopez-Benitez J,Tognoni CM,Carreras I,Dedeoglu A

PA1-1040 was used in Western Blotting to reveal that dysregulation of S1P and S1PR signaling may associate with the development of AD-like pathology through Akt/mTor/Tau signaling.

Sun Jan 15 00:00:00 UTC 2023

Novel delivery of cellular therapy to reduce ischemia reperfusion injury in kidney transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Thompson ER,Bates L,Ibrahim IK,Sewpaul A,Stenberg B,McNeill A,Figueiredo R,Girdlestone T,Wilkins GC,Wang L,Tingle SJ,Scott WE,de Paula Lemos H,Mellor AL,Roobrouck VD,Ting AE,Hosgood SA,Nicholson ML,Fisher AJ,Ali S,Sheerin NS,Wilson CH

PA1-1040 was used in Immunocytochemistry to report the first successful delivery of cellular therapy to a human kidney during NMP.

Thu Apr 01 00:00:00 UTC 2021

Novel delivery of cellular therapy to reduce ischemia reperfusion injury in kidney transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

PA1-1040 was used in Immunocytochemistry to report the first successful delivery of cellular therapy to a human kidney during NMP.

Thu Apr 01 00:00:00 UTC 2021

Inhibition of Sphingosine-1-Phosphate Receptor 2 by JTE013 Promoted Osteogenesis by Increasing Vesicle Trafficking, Wnt/Ca²⁺, and BMP/Smad Signaling.

International journal of molecular sciences

Lin S,Pandruvada S,Yu H

PA1-1040 was used in Western Blot to supported that enhanced osteogenesis in JTE013-treated BMSCs is associated with increased vesicle trafficking, which promotes the synthesis and transport of osteogenic protein and matrix vesicles and enhances matrix mineralization.

Mon Nov 08 00:00:00 UTC 2021

Inhibition of Sphingosine-1-Phosphate Receptor 2 by JTE013 Promoted Osteogenesis by Increasing Vesicle Trafficking, Wnt/Ca²⁺, and BMP/Smad Signaling.

International journal of molecular sciences

Lin S,Pandruvada S,Yu H

Mon Nov 08 00:00:00 UTC 2021

Silencing of Sphingosine kinase 1 Affects Maturation Pathways in Mouse Neonatal Cardiomyocytes.

International journal of molecular sciences

Jozefczuk E,Szczepaniak P,Guzik TJ,Siedlinski M

PA1-1040 was used in Western Blotting to demonstrate that the Sphk1 silencing in neonatal mouse CMs facilitated their postnatal maturation in both physiological and stress conditions.

Wed Mar 31 00:00:00 UTC 2021

Silencing of Sphingosine kinase 1 Affects Maturation Pathways in Mouse Neonatal Cardiomyocytes.

International journal of molecular sciences

Jozefczuk E,Szczepaniak P,Guzik TJ,Siedlinski M

PA1-1040 was used in Western Blotting to demonstrate that the Sphk1 silencing in neonatal mouse CMs facilitated their postnatal maturation in both physiological and stress conditions.

Wed Mar 31 00:00:00 UTC 2021

Dysregulation of sphingolipid metabolism contributes to bortezomib-induced neuropathic pain.

The Journal of experimental medicine

Stockstill K,Doyle TM,Yan X,Chen Z,Janes K,Little JW,Braden K,Lauro F,Giancotti LA,Harada CM,Yadav R,Xiao WH,Lionberger JM,Neumann WL,Bennett GJ,Weng HR,Spiegel S,Salvemini D

PA1-1040 was used in Western Blotting to study the mechanism by which bortezomib-induced painful peripheral neuropathy develops.

Mon May 07 00:00:00 UTC 2018

Sphingosine-1-Phosphate Receptor 1 Is Involved in Non-Obese Diabetic Mouse Thymocyte Migration Disorders.

International journal of molecular sciences

Lemos JP,Smaniotto S,Messias CV,Moreira OC,Cotta-de-Almeida V,Dardenne M,Savino W,Mendes-da-Cruz DA

Published figure using S1P1 polyclonal antibody (Product # PA1-1040) in Immunofluorescence

Sat May 12 00:00:00 UTC 2018

Sphingosine-1-Phosphate Receptor 1 Is Involved in Non-Obese Diabetic Mouse Thymocyte Migration Disorders.

International journal of molecular sciences

Lemos JP,Smaniotto S,Messias CV,Moreira OC,Cotta-de-Almeida V,Dardenne M,Savino W,Mendes-da-Cruz DA

Published figure using S1P1 polyclonal antibody (Product # PA1-1040) in Immunofluorescence

Sat May 12 00:00:00 UTC 2018

Sphingosine 1-Phosphate Receptor 1 Modulates CNTF-Induced Axonal Growth and Neuroprotection in the Mouse Visual System.

Neural plasticity

Joly S,Dalkara D,Pernet V

PA1-1040 was used in Immunohistochemistry-immunofluorescence to suggest that sphingosine 1-phosphate receptor 1 is a major regulator of retinal neuron survival and restricts the ciliary neurotrophic factor-induced retinal ganglion cell growth response.

Mon Jul 30 00:00:00 UTC 2018

Sphingosine 1-phosphate receptor 1 is required for retinal ganglion cell survival after optic nerve trauma.

Journal of neurochemistry

Joly S,Pernet V

PA1-1040 was used in immunocytochemistry, immunohistochemistry - frozen section, and western blot to assess the requirement for retinal ganglion cell survival after optic nerve trauma and sphingosine 1-phosphate receptor 1

Mon Aug 01 00:00:00 UTC 2016

Stress-induced production of chemokines by hair follicles regulates the trafficking of dendritic cells in skin.

Nature immunology

Nagao K,Kobayashi T,Moro K,Ohyama M,Adachi T,Kitashima DY,Ueha S,Horiuchi K,Tanizaki H,Kabashima K,Kubo A,Cho YH,Clausen BE,Matsushima K,Suematsu M,Furtado GC,Lira SA,Farber JM,Udey MC,Amagai M

PA1-1040 was used in immunohistochemistry to study the regulatory role of stress-induced chemokines on dendritic cell trafficking in skin

Sun Jun 24 00:00:00 UTC 2012

A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart.

Glycobiology

Wang S,Zhang Z,Lin X,Xu DS,Feng Y,Ding K

PA1-1040 was used in western blot to investigate the effect of the polysaccharide MDG-1 on specific gene expression and cell survival in ischemic hearts

Fri Jan 01 00:00:00 UTC 2010

Bioinformatics

Protein Aliases: CD antigen CD363; CD363; CD363 antigen; EDG1 (Edg1); Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation sphingolipid G-protein-coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; Lysophospholipid receptor B1; S1P receptor 1; S1P receptor Edg-1; S1P1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; sphingosine 1-phosphate receptor EDG1

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Gene Aliases: AI849002; CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; Lpb1; S1p; S1P1; S1PR1

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UniProt ID: (Human) P21453, (Rat) P48303, (Mouse) O08530

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Entrez Gene ID: (Human) 1901, (Rat) 29733, (Mouse) 13609

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Function(s)

G-protein coupled receptor binding G-protein coupled receptor activity sphingosine-1-phosphate receptor activity sphingolipid binding signal transducer activity G-protein coupled receptor

Process(es)

angiogenesis blood vessel maturation cardiac muscle tissue growth involved in heart morphogenesis sphingosine-1-phosphate signaling pathway chemotaxis cell adhesion G-protein coupled receptor signaling pathway adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway brain development cell migration transmission of nerve impulse lamellipodium assembly regulation of cell adhesion neuron differentiation positive regulation of cell migration regulation of bone mineralization actin cytoskeleton reorganization positive regulation of GTPase activity regulation of bone resorption endothelial cell differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle cell proliferation positive regulation of positive chemotaxis positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway negative regulation of stress fiber assembly heart trabecula morphogenesis T cell migration positive regulation of cell proliferation signal transduction

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S1P1 Polyclonal Antibody

S1P1 Antibody (PA1-1040) in ICC/IF

Product Details

PA1-1040

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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