Invitrogen

S1P1 Polyclonal Antibody

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S1P1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of EDG1 was performed using 70% confluent log phase Neuro-2a cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EDG1 Rabbit Polyclonal Antibody (Product # PA1-1040) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in the membrane. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

S1P1 Antibody in Western Blot (WB) — Western blot analysis of EDG1 was performed using tissue extract and whole cell lysate of Rat Brain (Lane 1) and Neuro- 2a (Lane 2). The blots were probed with Anti- EDG1 Rabbit polyclonal Antibody (Product # PA1-1040, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A ~ 42 kDa band corresponding to EDG1 was observed across tissue and cell line tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

S1P1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Published figure using S1P1 polyclonal antibody (Product # PA1-1040) in Immunohistochemistry (Frozen)

S1P1 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow) — Figure 1 Decreased expression of sphingosine-1-phosphate (S1P1) in non-obese diabetic (NOD) mouse thymocytes. Panel ( A ) shows the S1P1 expression in CD4/CD8-defined thymocyte subpopulations in C57BL/6 (black bars) and NOD (white bars) mice, analyzed by flow cytometry. The histograms show the S1P1 representative staining in NOD (white curve) and C57BL/6 (black curve) thymocyte subpopulations. Grey curves represent the negative staining control for C57BL/6 and the region marks the positive staining. Total = total thymocytes; DN = CD4 - CD8 - double-negative; DP = CD4 + CD8 + double-positive; CD4 + = CD4 + CD8 - single-positive; CD8 + = CD4 - CD8 + single-positive; MFI = median fluorescence intensity. Results are expressed as mean +- SEM and were analyzed by 2-way ANOVA followed by Tukey's post-test. Differences were considered statistically significant when *** or ### p < 0.001; **** or #### p < 0.0001. Asterisks represent statistical significance between C57BL/6 and NOD subpopulations; hash marks represent statistical significance between DP and SP subpopulations in the same mouse strain, after evaluating 3 C57BL/6 and 4 NOD thymi. ( B ) immunohistochemistry showing the S1P1, fibronectin (FN) and cytokeratin (CK) expression profile in NOD and S1P1 expression in C57BL/6 thymus sections. The white dashed lines delimit the giant PVS. C = cortical region; M = medullary region. Three thymi per group were evaluated, with 1 cryosection being analyzed. Original magnif

S1P1 Antibody in Immunohistochemistry (IHC) — Published figure using S1P1 polyclonal antibody (Product # PA1-1040) in Immunohistochemistry

Product Details

PA1-1040

Applications	Tested Dilution	Publications
Western Blot (WB)	2-4 µg/mL	View 4 publications 4 publications
Immunohistochemistry (IHC)	Assay-dependent	View 3 publications 3 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	2 µg/mL	View 1 publication 1 publication
Flow Cytometry (Flow)	-	View 1 publication 1 publication
Immunoprecipitation (IP)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 1mg/mL BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2184729

Product Specific Information

PA1-1040 detects S1P1 protein in transfected human and mouse cell samples. This antibody shows no cross-reactivity to S1P3.

PA1-1040 has successfully been used in immuno-fluorescence, immunohistochemistry, immunoprecipitation and Western blot procedures. By Western blot, this antibody detects an ~44 kDa protein representing recombinant S1P1 from transfected HEK293 cells. Immunofluorescence data demonstrates that S1P1 is localized to the plasma membrane after staining with PA1-1040.

The PA1-1040 immunogen is a synthetic peptide corresponding to residues S(359) H P Q K D D G D N P E T I(372) of mouse S1P1. This sequence is completely conserved in rat and 92% conserved in human S1P1. This peptide (Cat. # PEP-220) is available for use in neutralization and control experiments.

Target Information

S1P1 is a G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that is coupled to the G(i) subclass of heteromeric G proteins. S1P1 signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. It plays an important role in cell migration via its role in the reorganization of the actin cytoskeleton and formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. S1P1 is required for normal chemotaxis toward sphingosine 1-phosphate, normal embryonic heart development, and normal cardiac morphogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of Sphingosine-1-Phosphate Receptor 2 by JTE013 Promoted Osteogenesis by Increasing Vesicle Trafficking, Wnt/Ca²⁺, and BMP/Smad Signaling.

International journal of molecular sciences

Lin S,Pandruvada S,Yu H

PA1-1040 was used in Western Blot to supported that enhanced osteogenesis in JTE013-treated BMSCs is associated with increased vesicle trafficking, which promotes the synthesis and transport of osteogenic protein and matrix vesicles and enhances matrix mineralization.

Mon Nov 08 00:00:00 EST 2021

Novel delivery of cellular therapy to reduce ischemia reperfusion injury in kidney transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Thompson ER,Bates L,Ibrahim IK,Sewpaul A,Stenberg B,McNeill A,Figueiredo R,Girdlestone T,Wilkins GC,Wang L,Tingle SJ,Scott WE,de Paula Lemos H,Mellor AL,Roobrouck VD,Ting AE,Hosgood SA,Nicholson ML,Fisher AJ,Ali S,Sheerin NS,Wilson CH

PA1-1040 was used in Immunocytochemistry to report the first successful delivery of cellular therapy to a human kidney during NMP.

Thu Apr 01 00:00:00 EDT 2021

Novel delivery of cellular therapy to reduce ischemia reperfusion injury in kidney transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

PA1-1040 was used in Immunocytochemistry to report the first successful delivery of cellular therapy to a human kidney during NMP.

Thu Apr 01 00:00:00 EDT 2021

Silencing of Sphingosine kinase 1 Affects Maturation Pathways in Mouse Neonatal Cardiomyocytes.

International journal of molecular sciences

Jozefczuk E,Szczepaniak P,Guzik TJ,Siedlinski M

Published figure using S1P1 polyclonal antibody (Product # PA1-1040) in Western Blot

Wed Mar 31 00:00:00 EDT 2021

Sphingosine 1-Phosphate Receptor 1 Modulates CNTF-Induced Axonal Growth and Neuroprotection in the Mouse Visual System.

Neural plasticity

Joly S,Dalkara D,Pernet V

PA1-1040 was used in Immunohistochemistry-immunofluorescence to suggest that sphingosine 1-phosphate receptor 1 is a major regulator of retinal neuron survival and restricts the ciliary neurotrophic factor-induced retinal ganglion cell growth response.

Mon Jul 30 00:00:00 EDT 2018

Sphingosine-1-Phosphate Receptor 1 Is Involved in Non-Obese Diabetic Mouse Thymocyte Migration Disorders.

International journal of molecular sciences

Lemos JP,Smaniotto S,Messias CV,Moreira OC,Cotta-de-Almeida V,Dardenne M,Savino W,Mendes-da-Cruz DA

Published figure using S1P1 polyclonal antibody (Product # PA1-1040) in Flow Cytometry

Sat May 12 00:00:00 EDT 2018

Sphingosine-1-Phosphate Receptor 1 Is Involved in Non-Obese Diabetic Mouse Thymocyte Migration Disorders.

International journal of molecular sciences

Lemos JP,Smaniotto S,Messias CV,Moreira OC,Cotta-de-Almeida V,Dardenne M,Savino W,Mendes-da-Cruz DA

Published figure using S1P1 polyclonal antibody (Product # PA1-1040) in Flow Cytometry

Sat May 12 00:00:00 EDT 2018

Dysregulation of sphingolipid metabolism contributes to bortezomib-induced neuropathic pain.

The Journal of experimental medicine

Stockstill K,Doyle TM,Yan X,Chen Z,Janes K,Little JW,Braden K,Lauro F,Giancotti LA,Harada CM,Yadav R,Xiao WH,Lionberger JM,Neumann WL,Bennett GJ,Weng HR,Spiegel S,Salvemini D

PA1-1040 was used in Western Blotting to study the mechanism by which bortezomib-induced painful peripheral neuropathy develops.

Mon May 07 00:00:00 EDT 2018

Sphingosine 1-phosphate receptor 1 is required for retinal ganglion cell survival after optic nerve trauma.

Journal of neurochemistry

Joly S,Pernet V

PA1-1040 was used in immunocytochemistry, immunohistochemistry - frozen section, and western blot to assess the requirement for retinal ganglion cell survival after optic nerve trauma and sphingosine 1-phosphate receptor 1

Mon Aug 01 00:00:00 EDT 2016

Stress-induced production of chemokines by hair follicles regulates the trafficking of dendritic cells in skin.

Nature immunology

Nagao K,Kobayashi T,Moro K,Ohyama M,Adachi T,Kitashima DY,Ueha S,Horiuchi K,Tanizaki H,Kabashima K,Kubo A,Cho YH,Clausen BE,Matsushima K,Suematsu M,Furtado GC,Lira SA,Farber JM,Udey MC,Amagai M

PA1-1040 was used in immunohistochemistry to study the regulatory role of stress-induced chemokines on dendritic cell trafficking in skin

Sun Jun 24 00:00:00 EDT 2012

A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart.

Glycobiology

Wang S,Zhang Z,Lin X,Xu DS,Feng Y,Ding K

PA1-1040 was used in western blot to investigate the effect of the polysaccharide MDG-1 on specific gene expression and cell survival in ischemic hearts

Fri Jan 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: CD antigen CD363; CD363; CD363 antigen; EDG1 (Edg1); Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation sphingolipid G-protein-coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; Lysophospholipid receptor B1; S1P receptor 1; S1P receptor Edg-1; S1P1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; sphingosine 1-phosphate receptor EDG1

Gene Aliases: AI849002; CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; Lpb1; S1p; S1P1; S1PR1

UniProt ID: (Human) P21453, (Rat) P48303, (Mouse) O08530

Entrez Gene ID: (Human) 1901, (Rat) 29733, (Mouse) 13609

Molecular Function: G-protein coupled receptor