Invitrogen

S1P1 Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

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S1P1 Antibody in Immunocytochemistry (ICC/IF) — S1P1 Polyclonal Antibody detects EDG1 protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EDG1 protein stained by S1P1 Polyclonal Antibody (Product # PA5-85068) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

S1P1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded mouse brain, using EDG1 (Product # PA5-85068) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

S1P1 Antibody in Western Blot (WB) — Western Blot using S1P1 Polyclonal Antibody (Product # PA5-85068). Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with S1P1 Polyclonal Antibody (Product # PA5-85068) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Details

PA5-85068

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human EDG1. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2792217

Product Specific Information

Keep as concentrated solution.

Positive Control: HepG2, mouse liver.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

S1P1 is a G-protein coupled receptor for the bioactive lysosphingolipid sphingosine 1-phosphate (S1P) that is coupled to the G(i) subclass of heteromeric G proteins. S1P1 signaling leads to the activation of RAC1, SRC, PTK2/FAK1 and MAP kinases. It plays an important role in cell migration via its role in the reorganization of the actin cytoskeleton and formation of lamellipodia in response to stimuli that increase the activity of the sphingosine kinase SPHK1. S1P1 is required for normal chemotaxis toward sphingosine 1-phosphate, normal embryonic heart development, and normal cardiac morphogenesis.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD antigen CD363; CD363; CD363 antigen; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation sphingolipid G-protein-coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; Lysophospholipid receptor B1; S1P receptor 1; S1P receptor Edg-1; Sphingosine 1-phosphate receptor 1; Sphingosine 1-phosphate receptor Edg-1; sphingosine 1-phosphate receptor EDG1

Gene Aliases: AI849002; CD363; CHEDG1; D1S3362; ECGF1; EDG-1; EDG1; Lpb1; S1p; S1P1; S1PR1

UniProt ID: (Human) P21453, (Mouse) O08530

Entrez Gene ID: (Human) 1901, (Mouse) 13609

Molecular Function: G-protein coupled receptor