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Agrisera
S6K1/2/Ribosomal S6 kinase 1/2 Polyclonal Antibody
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FIGURE: 1 / 2
S6K1/2/Ribosomal S6 kinase 1/2 Antibody (AS12-1855) in WB
20 µg of total protein from flowers and leaves of transgenic Arabidopsis thaliana lines were analyzed (expressing the genomic copy of S 6 K 1 tagged with FLAG epitope under the control of its own promoter) extracted with homogenization buffer were separated on 10% SDS-PAGE and blotted 2 h to PVDF. Blots were blocked with 5% milk in TBST for 1 h at room temperature (RT) with agitation. Blot was incubated in ... View More
Product Details
AS12-1855
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide, derived from Arabidopsis thaliana.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
S6K1/2 encode closely related AGC-family serine/threonine kinases that act predominantly in the cytoplasm but can also be detected in the nucleus in an isoform- and phosphorylation-dependent manner. They are soluble proteins (no transmembrane segments) built around a conserved central protein kinase catalytic domain flanked by divergent N- and C-terminal regulatory regions; activation requires multisite phosphorylation, including hydrophobic-motif phosphorylation (classically at Thr389 in S6K1) that promotes the active kinase conformation and facilitates activation-loop phosphorylation by PDK1. Functionally, S6K1 and S6K2 are major downstream effectors of mTORC1 signaling: once activated, they phosphorylate ribosomal protein S6 and additional translation-related substrates such as eIF4B, thereby promoting ribosome biogenesis and selective translation programs (including 5prime TOP mRNAs), which drives anabolic growth, increased protein synthesis, and cell proliferation; consistent with this role, altered S6K signaling is frequently linked to growth control and cancer-relevant phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: KLS; p70 S6KA; P70(S6K)-ALPHA; p70(S6K)-beta; p70-alpha; P70-beta; P70-beta-1; P70-beta-2; p70-S6K; p70-S6K 2; p70S6Kb; PS6K; RPS6KB1; RPS6KB2; S6K; S6K-beta-1; S6K-beta-2; S6K-beta2; S6K1; S6K2; S6KB; S6Kbeta; S6KI(2); SRK; STK14A; STK14B
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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