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Invitrogen
Alpaca anti-Rabbit IgG Nano (VHH) Recombinant Secondary Antibody, Alexa Fluor™ 488
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FIGURE: 1 / 3
Rabbit IgG Nano (VHH) Secondary Antibody (SA5-10323) in ICC/IF
Immunofluorescence analysis in HeLa cells. Samples were incubated with Lamin B1 nuclear lamina (green) primary antibodies and followed by Rabbit IgG (VHH) secondary antibody (Product # SA5-10323), alpaca anti-rabbit IgG VHH Alexa Fluor 488. Scale bar: 20 µm.
Product Details
SA5-10323
Applications
Tested Dilution
Western Blot (WB)
1:1,000
Immunocytochemistry (ICC/IF)
1:1,000
Dot blot (DB)
Assay-dependent
Immunomicroscopy (IM)
1:1,000
Product Specifications
Host/Isotype
Alpaca
/ IgG
Expression System
E. coli
Class
Recombinant Monoclonal
Type
Secondary Antibody
Clone
CTK0101/CTK0102
Immunogen
Purified rabbit IgG
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
IgG
Antibody Form
VHH
Product Specific Information
Biclonal nanobodies specific for rabbit IgG Fab and Fc. CTK0101: KD = 0.2 nM, CTK0102: KD = 1.2 nM
Non Cross-Reactivity includes: Mouse, rat, sheep, goat, and guinea pig serum. Cross-reactivity to: Human and macaque serum
Rapid workflow: Simultaneous incubation of rabbit IgG primary antibody and Alpaca anti-rabbit IgG Nano (VHH) recombinant secondary antibody.
Offered through a partnership with ChromoTek.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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