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Invitrogen
Goat anti-Rat IgG2a Recombinant Secondary Antibody, Alexa Fluor™ Plus 555
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FIGURE: 1 / 3
Rat IgG2a Secondary Antibody (SA5-10376-AFP555) in ICC/IF
Immunofluorescence analysis of Goat anti-Rat IgG2a Recombinant Secondary Antibody, Alexa Fluor™ Plus 555 (Product # SA5-10376-AFP555) was performed on fixed and permeabilized LNCaP cells blocked with SuperBlock™ Blocking Buffer at room temperature and stained with alpha Tubulin Monoclonal Antibody (YL1/2) (Product # MA1-80017, 1:500) in SuperBlock™ Blocking Buffer (Product # 37515, 1:20) overnight at 4 degr... View More
Product Details
SA5-10376-AFP555
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
1:400
Immunocytochemistry (ICC/IF)
1:1,000
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Secondary Antibody
Clone
3H24L4
Immunogen
Purified Rat IgG
Conjugate
Alexa Fluor™ Plus 555
Alexa Fluor™ Plus 555
Alexa Fluor™ Plus 555
Excitation/Emission Max
558/572 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
250 µg
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Target
IgG2a
Antibody Form
Whole Antibody
Product Specific Information
Goat anti-Rat IgG2a Recombinant Secondary Antibody, Alexa Fluor™ Plus 555 is specific to Rat IgG2a. It has ≤ 5% cross reactivity to Mouse IgG and Rabbit IgG. It showed no cross-reactivity to various species immunoglobulins tested.
Alexa Fluor™ Plus dyes have been chemically optimized to provide higher signal to noise ratios and minimized cross-reactivity to help detect low-abundance targets in rare or precious samples.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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