Invitrogen

SACM1L Polyclonal Antibody

View all (6) SACM1L antibodies

Questions & Answers

Questions & Answers

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SACM1L Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SACM1L in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SACM1L polyclonal antibody (Product # PA5-106374) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

SACM1L Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded SACM1L in human lymph cancer tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with SACM1L polyclonal antibody (Product # PA5-106374) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

SACM1L Antibody in Western Blot (WB) — Western blot analysis of SACM1L in human kidney tissue (left lane: treated with blocking peptide). Samples were incubated with SACM1L polyclonal antibody (Product # PA5-106374).

Product Details

PA5-106374

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human SACM1L(Accession Q9NTJ5), corresponding to amino acid residues S279-Q302. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2854049

Target Information

Phosphoinositide phosphatase that hydrolyzes PtdIns(3)P and PtdIns(4)P. Has low activity towards PtdIns(3,5)P2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: phosphatidylinositide phosphatase SAC1; Phosphatidylinositol-3-phosphatase SAC1; Phosphatidylinositol-4-phosphate phosphatase; polyphosphatidylinositol phosphatase; suppressor of actin 1; Suppressor of actin mutations 1-like protein

Gene Aliases: KIAA0851; mKIAA0851; SAC1; Sac1p; SACM1L

UniProt ID: (Human) Q9NTJ5, (Mouse) Q9EP69, (Rat) Q9ES21

Entrez Gene ID: (Human) 22908, (Mouse) 83493, (Rat) 116482

Molecular Function: phosphatase hydrolase metabolite interconversion enzyme

phosphatidylinositol biosynthetic process biological_process phosphatidylinositol dephosphorylation

Process(es)

phosphatidylinositol-3-phosphatase activity protein binding phosphatase activity phosphatidylinositol bisphosphate phosphatase activity phosphatidylinositol phosphate 4-phosphatase activity phosphatidylinositol-4-phosphate phosphatase activity protein tyrosine/serine/threonine phosphatase activity protein tyrosine/threonine phosphatase activity JUN kinase phosphatase activity phosphohistidine phosphatase activity inositol bisphosphate phosphatase activity hydrolase activity MAP kinase tyrosine/serine/threonine phosphatase activity inositol-1,3,4-trisphosphate 4-phosphatase activity NADP phosphatase activity transmembrane receptor protein phosphatase activity inositol-1,3,4,5,6-pentakisphosphate 3-phosphatase activity inositol-1,4,5,6-tetrakisphosphate 6-phosphatase activity inositol-4,5-bisphosphate 5-phosphatase activity phosphoric ester hydrolase activity 5-amino-6-(5-phosphoribitylamino)uracil phosphatase activity phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity inositol phosphate phosphatase activity inositol-1,3,4,5,6-pentakisphosphate 1-phosphatase activity inositol-3,4-bisphosphate 4-phosphatase activity inositol-1,3,4-trisphosphate 1-phosphatase activity inositol-1,3,4,6-tetrakisphosphate 6-phosphatase activity inositol-1,3,4,6-tetrakisphosphate 1-phosphatase activity phosphatidylinositol-1,4,5-trisphosphate 5-phosphatase activity IDP phosphatase activity