Invitrogen

SACM1L Polyclonal Antibody

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SACM1L Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of SACM1L in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and SACM1L polyclonal antibody (Product # PA5-106374) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

SACM1L Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded SACM1L in human lymph cancer tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with SACM1L polyclonal antibody (Product # PA5-106374) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

SACM1L Antibody in Western Blot (WB) — Western blot analysis of SACM1L in human kidney tissue (left lane: treated with blocking peptide). Samples were incubated with SACM1L polyclonal antibody (Product # PA5-106374).

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human SACM1L, corresponding to a region within the internal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2854049

Target Information

Phosphoinositide phosphatase that hydrolyzes PtdIns(3)P and PtdIns(4)P. Has low activity towards PtdIns(3,5)P2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Phosphatidylinositide phosphatase SAC1; polyphosphatidylinositol phosphatase; suppressor of actin 1; Suppressor of actin mutations 1-like protein

Gene Aliases: KIAA0851; mKIAA0851; SAC1; Sac1p; SACM1L

UniProt ID: (Human) Q9NTJ5, (Mouse) Q9EP69, (Rat) Q9ES21

Entrez Gene ID: (Human) 22908, (Mouse) 83493, (Rat) 116482

Molecular Function: hydrolase phosphatase

Function(s)

phosphatidylinositol biosynthetic process biological_process phosphatidylinositol dephosphorylation

Process(es)

SACM1L Polyclonal Antibody

Product Details

Target Information

References (0)

Bioinformatics

Material safety data sheets (MSDS)

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