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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • SAE1 Antibodies

          Proteintech

          SAE1 Polyclonal Antibody

          4 References
          View all (27) SAE1 antibodies

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          Cite SAE1 Polyclonal Antibody

          • Antibody Testing Data (10)
          SAE1 Antibody in Immunocytochemistry (ICC/IF)
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          SAE1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          SAE1 Antibody (10229-1-AP) in ICC/IF

          Immunofluorescent analysis of HeLa cells using 10229-1-AP (SAE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SAE1 Antibody in Immunocytochemistry (ICC/IF)
          SAE1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SAE1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SAE1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SAE1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SAE1 Antibody in Western Blot (WB)
          SAE1 Antibody in Western Blot (WB)
          SAE1 Antibody in Western Blot (WB)
          SAE1 Antibody in Western Blot (WB)
          SAE1 Antibody in Western Blot (WB)
          SAE1 Polyclonal Antibody

          Product Details

          10229-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:200-1:1,000
          View 3 publications 3 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          SAE1 Fusion Protein Ag0275 (1-207 aa encoded by BC000344)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.22 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: MVEKEEAGG GISEEEAAQY DRQIRLWGLE AQKRLRASRV LLVGLKGLGA EIAKNLILAG VKGLTMLDHE QVTPEDPGAQ FLIRTGSVGR NRAEASLERA QNLNPMVDVK VDTEDIEKKP ESFFTQFDAV CLTCCSRDVI VKVDQICHKN SIKFFTGDVF GYHGYTFANL GEHEFVEEKT KVAKVSQGVE DGPDTKRAKL DSSETTMV (1-207 aa encoded by BC000344)

          Target Information

          Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2. form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999).

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: activator of SUMO1; FLJ3091; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; SUMO-1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; ubiquitin-like (sentrin) activating enzyme E1A; ubiquitin-like 1 (sentrin) activating enzyme E1A; ubiquitin-like 1 (sentrin) activating enzyme subunit 1; Ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme

          View more View less

          Gene Aliases: 2400010M20Rik; 2610044L12Rik; AL033372; AOS1; AW743391; D7Ertd177e; HSPC140; SAE1; SUA1; Ubl1a1; UBLE1A

          View more View less

          UniProt ID: (Human) Q9UBE0, (Mouse) Q9R1T2, (Rat) Q6AXQ0

          View more View less

          Entrez Gene ID: (Human) 10055, (Mouse) 56459, (Rat) 308384

          View more View less

          Function(s)
          ubiquitin activating enzyme activity protein binding protein C-terminus binding enzyme activator activity SUMO activating enzyme activity ATP-dependent protein binding protein heterodimerization activity small protein activating enzyme activity ligase activity ubiquitin-protein ligase
          Process(es)
          regulation of mitotic cell cycle protein ubiquitination protein sumoylation positive regulation of catalytic activity positive regulation of protein targeting to mitochondrion cellular protein modification process SMT3-dependent protein catabolic process
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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