|Tested species reactivity||Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues G(83) P V P P K P V P G K N T P K L N G(100) of rat SAP102.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||10 µg/ml|
|Western Blot (WB)||3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-045 detects synapse-associated protein 102 (SAP102) from rat tissues.
PA1-045 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~102 kDa protein representing SAP102 in rat brain homogenate. Immunohistochemical staining of SAP102 in rat hippocampus yields a pattern consistent with post-synaptic localization.
The PA1-045 immunogen is a synthetic peptide corresponding to residues G(83) P V P P K P V P G K N T P K L N G(100) of rat SAP102. PA1-045 immunizing peptide (Cat. # PEP-023) is available for use in neutralization and control experiments.
Synapse-Associated Protein 102 (SAP102) is one of a family of plasma membrane-associated proteins found in synaptic junctions. Like other members of the family, SAP102 has three ~90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity.
SAP102 is believed to participate in the clustering of certain proteins, including NMDA receptors, shaker-type potassium channels at the synaptic membrane in CNS neurons. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP102 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP102 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Increased [³H]D-aspartate release and changes in glutamate receptor expression in the hippocampus of the mnd mouse.
PA1-045 was used in western blot to study the role of Cln8 gene in hippocampal excitabilit and the glutamatergic transmission system
|Bigini P,Milanese M,Gardoni F,Longhi A,Bonifacino T,Barbera S,Fumagalli E,Di Luca M,Mennini T,Bonanno G||Journal of neuroscience research (90:1148)||2012|
NADPH oxidase-mediated oxidative damage to proteins in the postsynaptic density after transient cerebral ischemia and reperfusion.
PA1-045 was used in western blot to investigate the role of NADPH oxidase in the oxidative damage of postsynaptic density after transient cerebral ischemia and reperfusion
|Murotomi K,Takagi N,Takeo S,Tanonaka K||Molecular and cellular neurosciences (46:681)||2011|
Distinct levels of dopamine denervation differentially alter striatal synaptic plasticity and NMDA receptor subunit composition.
PA1-045 was used in western blot to investigate the effect of dopamine depletion on synaptic function and plasticity
|Paillé V,Picconi B,Bagetta V,Ghiglieri V,Sgobio C,Di Filippo M,Viscomi MT,Giampà C,Fusco FR,Gardoni F,Bernardi G,Greengard P,Di Luca M,Calabresi P||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:14182)||2010|
Guanylyl cyclase/PSD-95 interaction: targeting of the nitric oxide-sensitive alpha2beta1 guanylyl cyclase to synaptic membranes.
PA1-045 was used in western blot to investigate the interaction between the alpha2beta1 guanylyl cyclase isoform and PSD-95 in rat brain.
|Russwurm M,Wittau N,Koesling D||The Journal of biological chemistry (276:44647)||2001|
Clustering of neuronal potassium channels is independent of their interaction with PSD-95.
PA1-045 was used in immunocytochemistry to study the relationship between neuronal potassium channels and PSD-95.
|Rasband MN,Park EW,Zhen D,Arbuckle MI,Poliak S,Peles E,Grant SG,Trimmer JS||The Journal of cell biology (159:663)||2002|