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SAP130 Antibody (PA5-100858) in ICC/IF
Immunocytochemistry analysis of SAP130 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with SAP130 polyclonal antibody (Product # PA5-100858) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-100858
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human SAP130(Accession Q9H0E3), corresponding to amino acid residues V1019-V1048.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2850356
Product Specific Information
Antibody detects endogenous levels of total SAP130.
Target Information
SAP130 is a subunit of the histone deacetylase-dependent SIN3A corepressor complex (Fleischer et al., 2003).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 130 kDa Sin3-associated polypeptide; FLJ12761; Histone deacetylase complex subunit SAP130; mSin3A-associated protein 130; RSE1; SAP 130; SF3b130; Sin3-associated polypeptide p130; Sin3A associated protein; Sin3A associated protein 130; Sin3A associated protein 130kDa; Sin3A associated protein, 130kDa; Sin3A-associated protein, 130kDa; STAF130
Gene Aliases: 2610304F09Rik; 6720406D06; RGD1311657; SAP130
UniProt ID: (Human) Q9H0E3, (Mouse) Q8BIH0
Entrez Gene ID: (Human) 79595, (Mouse) 269003, (Rat) 307527
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